Registration Dossier
Registration Dossier
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EC number: 236-425-5 | CAS number: 13361-34-7
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2002
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-ethylhexyl cyanoacetate
- EC Number:
- 236-425-5
- EC Name:
- 2-ethylhexyl cyanoacetate
- Cas Number:
- 13361-34-7
- Molecular formula:
- C11H19NO2
- IUPAC Name:
- 2-ethylhexyl 2-cyanoacetate
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Suspended matter: 28.2 mg/L
- Dry matter: 4.23 g/L
- Colony forming units (CFU): 114 x 10^4/mL - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 22.3 mg/L
- Based on:
- test mat.
- Details on study design:
- TEST CONDITIONS
- Test temperature: 21.0°C
TEST SYSTEM
- Culturing apparatus: 5000 mL Woulff flasks
- Number of culture flasks/concentration: 2 vessels without test item with inoculum
1 vessel containing control item and inoculum
2 vessels containing test item and inoculum
- Measuring equipment: Carbon analyser (Manufacturer Shimadzu)
SAMPLING
- Sampling frequency: 0, 2, 5, 9, 14, 19, 22, 28 and 29 days
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 0 d
- Remarks on result:
- other: mean of 2 vessels
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 51
- Sampling time:
- 5 d
- Remarks on result:
- other: mean of 2 vessels
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 105
- Sampling time:
- 14 d
- Remarks on result:
- other: mean of 2 vessels
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 104
- Sampling time:
- 22 d
- Remarks on result:
- other: mean of 2 vessels
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 107
- Sampling time:
- 28 d
- Remarks on result:
- other: mean of 2 vessels
- Details on results:
- The degradation-values >100% are due to methamatical calculations.
The 10 % level was reached at day 3.
BOD5 / COD results
- Results with reference substance:
- Kinetic of control substance:
45% after 2 days, 101% after 14 days, 97% after 22 days, 105% after 28 days
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Since 2-Ethylhexyl cyanoacetate (EHCA) is degraded by >60 % in the course of 10 days after the time when biodegradation had achieved 10 %,
2-Ethylhexyl cyanoacetate (EHCA) is considered as "readily biodegradable".
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