Disodium 6-hydroxy-5-[(4-sulphonatophenyl)azo]naphthalene-2-sulphonate

Endpoint summary

Additional information

Short-term toxicity to fish

No. of studies were reviewed for Short-term toxicity to fish from reliable sources having Klimisch rating 2 and 4.

The summary of the results are presented below   

Sr.No	Endpoint	Effect values	Basis for effects	Duration	Species	CAS No.	Sources
1.	LC50	216.465mg/L	Mortality	96 hrs.	Lepomis macrochirus	Target :2783-94-0	Predicted data from QSAR
2.	LC50	> 10000 mg/L	Mortality	96 hrs.	Fish	RA CAS: 25956-17-6	Experimental data from publication and study report.
		>1000 mg/L	Mortality	72 hrs.
		200 mg/L	Mortality	48 hrs.
3.	LC50	165 mg/L	Mortality	96 hrs.	Pimephales promelas	RA CAS: 633-96-5	Experimental data from study report.

 

Based on the studies summarized in the above table for the target substance disodium 6-hydroxy-5-[(4-sulphonatophenyl)azo]naphthalene-2-sulphonate and the read across substance, the endpoint value was found to vary between LC50 = 165 mg/L to > 10000 mg/L in a 96 hrs.,at 72 hrs. LC50 = >1000 mg/L and at 48 hrs. LC50 =200 mg/L; on the basis of mortality effect. Based on the above weight of evidence values, the substance it is concluded that the test substance dose not qualifies for the aquatic toxicity.

Long-term toxicity to fish

According to column I of annex IX, Long-term toxicity testing on fish need not to be conducted as it is already provide as part of Annex VIII requirements. Therefore this study was considered to be waived.

Short-term toxicity to aquatic invertebrates

No. of studies were reviewed for Short-term toxicity to aquatic invertebrate from reliable sources having Klimisch rating 2 and 4. 

The summary of the results are presented below   

Sr.No	Endpoint	Effect values	Basis for effects	Duration	Species	CAS No.	Sources
1.	EC50	382.262 mg/L	Intoxication	48 hrs.	Daphnia magna	Target cas no.2783-94-0	Predicted data from QSAR
2.	EC50	486.5mg/L	-	48 hrs.	Daphnia magna	Target cas no.2783-94-0	Predicted data from ECOSAR
3.	LC50	295 mg/L	-	48 hrs.	Daphnia magna	Read across substance cas no.25956-17-6	Predicted data from ECOSAR

 

Based on the studies summarized in the above table for the target substance disodium 6-hydroxy-5-[(4-sulphonatophenyl)azo]naphthalene-2-sulphonate and the read across substance, the endpoint value was found to be EC50 = 382.262 mg/L to486.5mg/L and LC50=295 mg/L in a 48 hrs.; on the basis of Intoxication effect. Based on the above weight of evidence values, the substance it is concluded that the test substance dose not qualifies for the aquatic toxicity. 

Long-term toxicity to aquatic invertebrates

According to column I of annex IX ; Long-term toxicity testing on invertebrate (species : Daphnia magna) need not to be conducted as it is already provided as a part of Annex VII requirements.Therefore this end point was considered for waiver.

Toxicity to aquatic algae and cyanobacteria

No. of studies were reviewed for toxicity to aquatic algae from reliable sources having Klimisch rating 2 and 4. 

The summary of the results are presented below   

Sr.No	Endpoint	Effect values	Basis for effects	Duration	Species	CAS No.	Sources
1.	NOEC	15.7290mg/L	Population	72 hrs.	Selenastrum capricornutum (new name: Pseudokirchnerella subcapitata)	Target cas no.2783-94-0	Predicted data from QSAR
2.	EC50	146000mg/L	-	96hrs.	Green algae	Target cas no.2783-94-0	Predicted data from ECOSAR
3.	EC50	44524 mg/L	-	96hrs.	Green algae	Read across substance cas no.25956-17-6	Predicted data from ECOSAR

 

Based on the studies summarized in the above table for the target substance disodium 6-hydroxy-5-[(4-sulphonatophenyl)azo]naphthalene-2-sulphonate and the read across substance, the endpoint value was found to vary between EC50 = 44524 mg/L to 146000mg/L and NOEC =15.7290 mg/L in a 72 hrs.and 96 hrs.; on the basis of Population effect. Based on the above weight of evidence values, the substance it is concluded that the test substance dose not qualifies for the aquatic toxicity.  

Toxicity to microorganisms

No. of studies were reviewed for toxicity to microorganisms from reliable sources having Klimisch rating 2 and 4.

The summary of the results are presented below    

Sr.No	Endpoint	Effect values	Basis for effects	Duration	Species	Sources
1.	NR-ZERO	0.325mg/L	Mortality	0.3333333 hrs.	Paramecium caudatum	Predicted data from QSAR
2.	IGC50	17.606mg/l	growth inhibition	46 hrs.	Tetrahymena pyriformis	Predicted data from Danish QSAR
3.	EC50	22.1 mg/L	inhibition of light output	5 min	Vibrio fisheri	Experimental data from Journal of Biotechnology

 

 Based on the studies summarized in the above table for the target substance disodium 6-hydroxy-5-[(4-sulphonatophenyl)azo]naphthalene-2-sulphonate, the endpoint value was found to be EC50 = 22.1 mg/L; IGC50 = 17.606mg/l and NR-ZERO =0.325mg/L in 5 min. to 46 hrs. Study on the basis of inhibition of light output, growth inhibition and Mortality effect.