O,O-diisopropyl hydrogen dithiophosphate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2003

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DS/EN ISO 14669 (1999)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

O,O-diisopropyl hydrogen dithiophosphate

EC Number:

203-503-5

EC Name:

O,O-diisopropyl hydrogen dithiophosphate

Cas Number:

107-56-2

Molecular formula:

C6H15O2PS2

IUPAC Name:

O,O-bis(propan-2-yl) sulfanylphosphonothioate

Test material form:

other: Liquid

Details on test material:

- Analytical purity: 98 % w/w

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations:(nominial) 0, 2, 5, 10, 20, 50, 100 and 200 mg/l

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

other aquatic crustacea: Acartia tonsa

Details on test organisms:

TEST ORGANISM
- Common name: Calanoid copepod (marine)
- Strain: Kept in culture at DHI since 1987, originally collected in the North Sea by Danmarks Fiskeriundersøgelser

Study design

Test type:

static

Water media type:

saltwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

19 ± 0.5 ºC

pH:

8.0 ± 0.2

Dissolved oxygen:

≥ 4 mg/l at test end

Salinity:

33 ‰

Nominal and measured concentrations:

Nominal concentrations: 0, 2, 5, 10, 20, 50, 100 and 200 mg/

Details on test conditions:

TEST SYSTEM
- Test vessels: 50 ml glass beakers with approx. 25 ml of test soulution

- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates):


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Natural seawater, filtered, collected in the North Sea near Hirtshals by Danmarks Fiskeriundersøgelser 26.10.2009
-

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 (light:dark)

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol, CAS No. : 591-35-5

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

ca. 105 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC10

Effect conc.:

> 10 - < 20 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- Mortality: yes
- LC50: 1.64 mg/l (a little higher than validation criterium, but not considered to have had significant influence on the test systems sensitivity

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

LC50(48 h) of O,O-diisopropyl hydrogen dithiophosphate, CAS No.107-56-2, is 105 mg/L for Acatia tonsa
Control group mortality was a little higher than validation criterium, but this is not considered to have had significant influence on the test result.