Registration Dossier
Registration Dossier
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EC number: 801-694-5 | CAS number: 1392325-86-8
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
The substance is not readily biodegradable in an OECD TG 301B test.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
The biodegradability of the substance is tested in a CO2 Evolution Test according to OECD TG 301B and in compliance with GLP (Harlan, 2014). Test substance at a concentration of 5 mg/L (based on carbon) is inoculated 28 days with activated sewage sludge from an STP treating predominantly domestic sewage. During the 28 -day incubation period, carbon dioxide production is measured and biodegradation determined as % of ThCO2. After the 28-d incubation period, biodegradation is 0%. The reference substance sodium benzoate attained 84% biodegradation after 14 days and 90% biodegradation after 28 days thereby confirming the suitability of the inoculum and test conditions. The toxicity control attained 47% biodegradation after 14 days and 66% biodegradation after 28 days thereby confirming that the test item did not exhibit an inhibitory effect on the inoculum. Therefore, the substance is not readily biodegradable.
In a supporting study, the biodegradability of the substance was examined in a Manometric Respirometry Test according to OECD TG 301F and in compliance with GLP (Sinochem, 2013). The substance was added directly to the test vessels at a concentration of 36.6 mg/L (equivalent to 98.3 mg ThOD/L), and inoculated for 28 days with activated sludge from a domestic sewage. During inoculation the oxygen consumption was recorded and the biodegradation determined as % of the theoretical oxygen demand (ThOD).
The percentage biodegradation of test substance averaged 3.6% (n=2) after 28 days. Biodegradation of the reference substance attained 61.3% by Day 7, 78.3% by Day 14 and 87.1% by Day 28, thereby confirming the suitability of the inoculum and test conditions. In the toxicity control, 36.3% degradation occurred within 14 days. Since this value exceeded the 25% ThOD limit for (test item) toxicity, it was concluded that the test item was not inhibitory to sludge micro-organisms under the described conditions of this test. Therefore, the substance is not readily biodegradable.
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