Methyl [(dimethoxyphosphinothioyl)thio]acetate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 01-10-1992 to 28-10-1992

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

no

GLP compliance:

yes

Test type:

acute toxic class method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Methyl [(dimethoxyphosphinothioyl)thio]acetate

EC Number:

212-058-6

EC Name:

Methyl [(dimethoxyphosphinothioyl)thio]acetate

Cas Number:

757-86-8

Molecular formula:

C5H11O4PS2

IUPAC Name:

methyl 2-{[dimethoxy(sulfanylidene)-λ⁵-phosphanyl]sulfanyl}acetate

Details on test material:

- Name of test material (as cited in study report): MPEM
- Substance type: Intermediate
- Physical state: Liquid
- Purity test date:
- Lot/batch No.: 20825

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Møllegaard Breeding Centre Ltd, Ejby, DK-4623 Ll. Skensved, Denmark
- Age at study initiation: 6-7 weeks
- Weight at study initiation: 134-164 g
- Fasting period before study: -
- Housing: Macrolone cages type III, 2 or 3 to a cage, males and females separated. The bedding was pinewood sawdust "Linocel Granulat" from Compagny Altromin GmbH D-4937 Lage, Lippe, Germany
- Diet : "Altromin 1314" from Compagny Altromin GmbH D-4937 Lage, Lippe, Germany
- Water : ad libitum, acidified with hydrochloric acid to pH 2,5.
- Acclimation period: 5 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21, ± 3
- Humidity (%):55, ± 15
- Air changes (per hr): 10 times
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

peanut oil

Details on oral exposure:

VEHICLE
- Concentration in vehicle:-
- Amount of vehicle (if gavage): 10 ml/kg b.w.
- Justification for choice of vehicle:
- Lot/batch no. (if required): 20825
- Purity:-



CLASS METHOD (if applicable)
- Rationale for the selection of the starting dose: range finding showed LD50 over 2000 mg/kg b.w., therefore this was chosen chosen for only one group

Doses:

2000 mg/kg b.w.

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Observations: 3 times the first day, thereafter daily
Weighing: daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,organ weights, histopathology, other:

Results and discussion

Preliminary study:

It was expected that the LD50 would exceed 2 g test material/kg body weight. Therefore the study was initiated with one group consisting of 5 males and 5 females, which all were adnistered 2000 mg methyl [(dimethoxyphosphinothioyl)thio]acetate per kg of body weight.

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Based on:

test mat.

Mortality:

None

Clinical signs:

other: Piloerection and pinched abdomen on day of treatment, piloerection day 1-4. from day 5 and onwards no symptoms

Gross pathology:

None

Other findings:

None

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

Since no rats died of the treatment, the oral LD50 of methyl [(dimethoxyphosphinothioyl)thio]acetate must exceed 2000 mg/kg body weight.