3-methyl-2-butenal

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Principles of method if other than guideline:

Before OECD Guideline 404 was established in 1982, skin irritation was tested using an internal method (BASF test).

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-methyl-2-butenal

EC Number:

203-527-6

EC Name:

3-methyl-2-butenal

Cas Number:

107-86-8

Molecular formula:

C5H8O

IUPAC Name:

3-methyl-2-butenal

Details on test material:

- Name of test material (as cited in study report): 3-Methyl-2-butenal, CAS: 107-86-8
- Analytical purity: 98%

Test animals

Species:

rabbit

Strain:

Vienna White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Gaukler
- Weight at study initiation: 2,81- 3.41 kg

Test system

Type of coverage:

occlusive

Preparation of test site:

not specified

Vehicle:

unchanged (no vehicle)

Controls:

other: untreated skin of the same animal

Amount / concentration applied:

undiluted

Duration of treatment / exposure:

4 hour(s)

Observation period:

7 d

Number of animals:

2

Details on study design:

TEST SITE
- Area of exposure: 2.0 x 2.0 cm
- % coverage: 100
- Type of wrap if used: plastic film


REMOVAL OF TEST SUBSTANCE
- Washing (if done): Lutrol
- Time after start of exposure: 3 min, 1h, 4h


SCORING SYSTEM:

Erythema:
1 questionable
2 slightly
3 strong
4 severe
Oedema:
1 very slightly
2 slightly
3 strong
4 severe

S= scaling, sS= strong scaling, N=necrosis, w=weak, p=parchmenty, l=leather-like,
h=indurated slidable, t=penetrative, fn=spotted necrotic, Na=cicatris, ü= extending the site of application, id=indurated, an=anaemic

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

mean

Time point:

other: 24 + 48 h

Score:

3

Max. score:

3

Reversibility:

not reversible

Results 2

Irritation parameter:

edema score

Basis:

mean

Time point:

other: 24 + 48 h

Score:

3.25

Max. score:

4

Reversibility:

not reversible

Any other information on results incl. tables

Readings	Animal	Exposure period: 4 h
		Erythema	Edema	Additional findings
				Erythema	Edema
4 h	1	2	3	ü
	2	3	4	ü
24 h	1	3	3
	2	3	4	ü, fl
48 h	1	3	3	ü, fl
	2	3	3	ü, fl
8 d	1	4	1	Nl, ü
	2	4	1	Nl, ü
Mean 24h, 48h,	1	3	3
	2	3	3.5
Mean	1-2	3	3.25

Readings	Animal	Exposure period: 1 h
		Erythema	Edema	Additional findings
				Erythema	Edema
4 h	1	*	3	b,ü
	2	4	3	Nw
24 h	1	4	4	Nw,ü	ü
	2	4	4	Nw,ü	ü
48 h	1	4	4	Nw,ü	ü
	2	4	4	Np,ü	ü
8 d	1	4	2	Np
	2	4	2	Nl, ü
Mean 24h, 48h,	1	4	4
	2	4	4
Mean	1-2	4	4

Readings	Animal	Exposure period: 3 min
		Erythema	Edema	Additional findings
				Erythema	Edema
4 h	1	*	0
	2	*	0
24 h	1	1	0
	2	1	0
48 h	1	1	0
	2	1	0
8 d	1	0	0	S
	2	1	0	S
Mean 24h, 48h,	1	1	0
	2	1	0
Mean	1-2	1	0

Symbols:

N  - necrosis

ü   - oedema/redness extending the site of application

l    - leather-like

p   - parchmenty

fl   - blotty necrotic

w  - weak

*   - reeding not possible due to substance related dying of the skin

Applicant's summary and conclusion

Interpretation of results:

corrosive

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

Edema, erythema and necrosis of the skin were seen as early
as 1 h after treatment and persisted until the end of the
observation period on day 8.

Based on this finding no additional eye irritation test was
performed.