Dodecanedioic acid, 1,12-bis[2-[4-(4,6-diphenyl-1,3,5-triazin-2-yl)-3-hydroxyphenoxy]ethyl] ester

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

28th January 2013 - 5th February 2013

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study conducted in a GLP compliant laboratory according to accepted regulatory study test guidelines

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Report date:

2013

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: "Method for Testing the Biodegradability of Chemical Substances by Microorganisms" stipulated in the "Testing Methods for New Chemical Substances" (March 31, 2011, No. 0331-7, Pharmaceutical and Food Safety Bureau, Ministry of Health, Labour and Welfare

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

1,12-bis({2-[4-(4,6-diphenyl-1,3,5-triazin-2-yl)-3-hydroxyphenoxy]ethyl}) dodecanedioate

EC Number:

939-042-8

Cas Number:

1482217-03-7

Molecular formula:

C58H56N6O8

IUPAC Name:

1,12-bis({2-[4-(4,6-diphenyl-1,3,5-triazin-2-yl)-3-hydroxyphenoxy]ethyl}) dodecanedioate

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

- Name of test material (as cited in study report): T-1620L
- Physical state: Pale yellow powder
- Analytical purity:99.9%
- Lot/batch No.: OF1211
- Storage condition of test material: Room temperature in the dark

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Appearance Light yellow powder
- Source and lot/batch number of test material: OF1211
- Purity: 99.9%

Study design

Inoculum or test system:

mixture of sewage, soil and natural water

Details on inoculum:

On-site sludge sampling was carried out at 10 locations in Japan (samples were from surface water and surface soil of rivers, lake and inland sea; return sludges from sewage plants). Activated sludge, which was prepared and controlled in this laboratory according to the test method, was used in this study (sampling period: November, 2012, initiation date of use: December 20, 2012). The activated sludge, which was cultivated for 19.5 hours after the synthetic sewage was added, was used. The synthetic sewage was prepared according to the following method; glucose, peptone, and potassium dihydrogenphosphate were dissolved in purified water, and the pH of the solution was adjusted to 7.0±1.0.
Concentration of activated sludge 30 mg/L (as the concentration of suspended solid)

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

28 d

BOD5 / COD results

BOD5 / COD

Parameter:

BOD5

Value:

0 mg O2/g test mat.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The average percentage biodegradation by BOD and that of the test item were 0%, respectively. In addition, no peak corresponding to the converted product was detected on the HPLC chromatograms. On the basis of these results, the test item was not biodegraded and remained in the test solutions
under the test conditions of this study.

Executive summary:

The test item was not biodegraded under the test conditions of this study.