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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Skin sensitisation

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Administrative data

Endpoint:
skin sensitisation: in vivo (non-LLNA)
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: The purity of the test substance was unknown. The protocol was not described in details.

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Sensitization to nordihydro-guaiaretic acid.
Author:
Golberg L.
Year:
1963
Bibliographic source:
Food and Cosmetics Toxicology, 1, 114.
Reference Type:
publication
Title:
Studies on allergy towards some simple chemical substances
Author:
Griepentrog F.
Year:
1961
Bibliographic source:
Arzneimittel-Forsch., 11, 920-922.

Materials and methods

Principles of method if other than guideline:
Guinea-pigs were injected with the test substance intradermally on 10 successive days. Three weeks after the injections, the test substance was
rubbed onto the injection site in the form of a 1% solution or suspension in peanut oil.
GLP compliance:
not specified
Type of study:
other: sensitization test
Justification for non-LLNA method:
Study conducted prior to the recognition of the LLNA test as the preferred in vivo test method to assess skin sensitisation.

Test material

Constituent 1
Chemical structure
Reference substance name:
Guaiacol
EC Number:
201-964-7
EC Name:
Guaiacol
Cas Number:
90-05-1
Molecular formula:
C7H8O2
IUPAC Name:
Phenol
Details on test material:
The origin of guaiacol was not reported and its purity was unknown.

In vivo test system

Test animals

Species:
guinea pig
Strain:
not specified
Sex:
not specified

Study design: in vivo (non-LLNA)

Induction
Route:
intradermal
Vehicle:
peanut oil
Concentration / amount:
1% in peanut oil
Day(s)/duration:
10 days
Adequacy of induction:
not specified
Challenge
No.:
#1
Route:
other: substance rubbed onto injection sites
Vehicle:
peanut oil
Concentration / amount:
1% in peanut oil
Day(s)/duration:
single administration
Adequacy of challenge:
not specified
No. of animals per dose:
no data
Details on study design:
Guinea-pigs were injected with the test substance intradermally on 10 successive days. Three weeks after the injections, the test substance was
rubbed onto the injection site in the form of a 1% solution or suspension in peanut oil and the area was examined 1, 2 and 3 days following the
application

Results and discussion

In vivo (non-LLNA)

Resultsopen allclose all
Reading:
1st reading
Group:
test chemical
Dose level:
not specified
No. with + reactions:
0
Remarks on result:
no indication of skin sensitisation
Reading:
1st reading
Group:
negative control
Dose level:
Not specified
No. with + reactions:
0
Remarks on result:
other: no data available
Reading:
1st reading
Group:
positive control
Dose level:
not specified
Remarks on result:
other: No data available

Any other information on results incl. tables

Guaiacol did not evoke skin reactions.

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Conclusions:
Not sensitizing
Executive summary:

In a sensitizing study (Goldberg, 1963), guinea-pigs were injected with Gaiacol intradermally on 10 successive days. Three weeks after the injections, the test substance was rubbed onto the injection site in the form of a 1% solution or suspension in peanut oil and the area was examined 1, 2 and 3 days following the application.

Gaiacol did not induce sensitization, and it should not be classified.