3,3',5,5'-tetra-tert-butylbiphenyl-2,2'-diol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study; testing period was 24 hours, a solubiliser was used

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3,3',5,5'-tetra-tert-butylbiphenyl-2,2'-diol

EC Number:

407-920-5

EC Name:

3,3',5,5'-tetra-tert-butylbiphenyl-2,2'-diol

Cas Number:

6390-69-8

Molecular formula:

C28 H42 O2

IUPAC Name:

3,3',5,5'-tetra-tert-butyl-[1,1'-biphenyl]-2,2'-diol

Details on test material:

- Name of test material (as cited in study report): CA 16-178
- Lot/batch No.: A 88103/Z1572R
- Purity: Lab. sample

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution was prepared by mixing 200 mg test substance with 8 mg alkylphenol-polyglykol-ether. The solution was made up to 2000 mL with water. The test solutions were prepared separately.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): alkylphenol-polyglykol-ether
- Concentration of vehicle in test medium (stock solution and final test solution): 4 mg/L alkylphenol-polyglykol-ether
- Evidence of undissolved material (e.g. precipitate, surface film, etc): Small parts of the test substance were swimming at the surface of the test
solution at cone. 5.8-100 mg/L nominal from the start of the test. A slight deposit was observed at conc. 32-100 mg/L nominal after 24 h exposure.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: bred at testing facility
- Age at study initiation (mean and range, SD): < 24 h
- Feeding during test: none

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Test conditions

Hardness:

240 mg CaCO3/L

Test temperature:

20 ± 1 °C

pH:

7.5 - 7.6

Dissolved oxygen:

94 - 96%

Nominal and measured concentrations:

Nominal test concentrations:
control, solvent control, 3.2, 5.8, 10, 18, 32, 58, 100 mg/L

Measured test concentrations:
at test start: 3.12, 5.77, 9.48, 16.63, 30.58, 57.38, 92.65 mg/l

at test termination: 2.55, 3.79, 7.80, 11.75, 28.31, 51.73, 94.51 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beakers covered with watch glasses
- Fill volume: 100 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted bidistilled water


OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day night regime
- Light intensity: Fluorescent light, approx. 2000 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 24 hours

Results and discussion

Effect concentrations

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility