6-methyloct-5-en-2-one

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions.

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference 1

Reference Type:

other company data

Title:

Unnamed

Year:

1974

Report date:

1974

Reference 2

Reference Type:

secondary source

Title:

No information

Author:

BASF AG

Year:

2008

Bibliographic source:

RIFM database

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Principles of method if other than guideline:

Study was performed acc. internal BASF method, which was in large part similiar to OECD 404

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

6-methylhept-5-en-2-one

EC Number:

203-816-7

EC Name:

6-methylhept-5-en-2-one

Cas Number:

110-93-0

IUPAC Name:

6-methylhept-5-en-2-one

Details on test material:

- Name of test material (as cited in study report): 6-Methyl-5-hepten-2-one,
- Analytical purity: 98 %
- pH = 5.6

Test animals

Species:

rabbit

Strain:

Vienna White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: M. Gaukler, Offenbach, Germany)
- Weight at study initiation: 2.98 and 2.93 (male), 2.47 and 2.92 kg (female)

Test system

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): ca. 0.5 ml
- Concentration (if solution): 100%

Duration of treatment / exposure:

1 min, 5 min, 15 min, 20 h

Observation period:

After exposure (only 1, 5, 15 min.), 24 hrs, 48 h, 5 days, 6 days and 8 days

Number of animals:

toal 4; 2 male, 2 female

Details on study design:

TEST SITE
- Area of exposure: 2.5 cm x 2.5 cm of back skin
- Type of wrap if used: cotton pad (seize: 2.5 cm x 2.5 cm) saturated with the undiluted test substance (ca. 0.5 ml)


REMOVAL OF TEST SUBSTANCE
- Washing (if done): 50% Lutrol (polyethylenglycol) dilution in water
- Time after start of exposure: 1, 5, 15 min and 20 h


SCORING SYSTEM:
Erythema and edema were scored according the below described system:
Although the results in the report were originally not given as Draize scores the data can be transferred into the Draize scoring system (in brackets). (+) = none - negligible effect (0)
+ = slight effect (1)
++ = moderate effect (2)
+++ = severe effect (>= 3)
N = necrosis

Results and discussion

Any other information on results incl. tables

LOCAL EFFECTS
Application of test substance for 1 min - 15 min did not lead to any skin findings. 
Application for 20 hrs led to questionable to slight redness  (score = (+) ) after 24 hrs which was fully reversible in all animals within 48
h, signs of edema were not observed, a slight scaling occurred in 1  animal. 

SYSTEMIC TOXICITY
There were no signs of clinical toxicity from the dermal exposure.
No mortality occurred. 

 

 

Applicant's summary and conclusion

Interpretation of results:

not irritating

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

Occlusive application of 6-methyl-hept-5-en-2-one for 20 h to rabbit skin lead to slight signs of irritation which were almost reversible within 48 hrs. Although a slight scaling remains after 8 days in only one animal, this finding is not sufficient for classification.