Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 269-125-8 | CAS number: 68187-80-4
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
A well conducted study with the structurally similar amides, C8-18 and C18-unsatd., N, N-bis(hydroxyethyl) conducted in Pseudomonas putida (bacteria) resulted in EC10 and EC50 values of 0.83 and 6 g/L respectively. Inhibition of cell growth was observed at 1.0 mg/L. Based on read-across these values will be used for the assessment of HE Rape Oil, reaction product with diethanolamine.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 6 g/L
- EC10 or NOEC for microorganisms:
- 0.83 g/L
Additional information
A study was conducted to assess the toxicity of the structurally similar amides, C8 -18 and C18 -unsatd., N, N-bis(hydroxyethyl) to the bacteria Pseudomona putida according to DIN guideline 38412 L8. Pseudomonas putida colonies, in triplicate, were exposed to the test substance at nominal concentrations of 0.01, 0.05, 0.10, 0.50, 1.00, 2.50, 5.00 and 10.00 g/L. Inhibition of cell growth compared to controls was recorded after 72 hours. The EC10was 0.83 g/L and the EC506.0 g/L. Inhibition of cell growth was observed as of 1.0 g/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
