Registration Dossier
Registration Dossier
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EC number: 601-495-1 | CAS number: 117829-74-0
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Qualifier:
- according to guideline
- Guideline:
- other: EEC Directive 88/302/EEC (Activated Sludge Respiration Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 3-(dimethylamino)propan-1-ol hydrochloride
- EC Number:
- 601-495-1
- Cas Number:
- 117829-74-0
- Molecular formula:
- C5 H13 N O. Cl H
- IUPAC Name:
- 3-(dimethylamino)propan-1-ol hydrochloride
- Details on test material:
- - Name of test material (as cited in study report): 3-Dimethylamino-Propanol-Hydrochlorid-1
- Batch no.: KT 215
- Product no.: 63181
- Aggregated state: liquid
- Colour: colourless-clear
- Homogeneity: yes
- Storage conditions: ambient
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not required
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Inoculum: activated sludge from laboratory wastewater plant treating municipal sewage. Concentration of dry substance 1 g/L
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 30 min
Test conditions
- Test temperature:
- 20 +/- 2 °C
- pH:
- TEST SUBSTANCE
- pH-value before adding inoculum (after correction): 7.3
- pH-value after incubation time of 30 min: 7.6
REFERENCE SUBSTANCE
- pH-value before adding inoculum (after correction): 7.1 - 7.3 - Dissolved oxygen:
- TEST SUBSTANCE
- oxygen concentration at start (mg O2/L): 7
- Oxygen concentration after 6 min (mg O2/L): 4.5
REFERENCE SUBSTANCE
- oxygen concentration at start (mg O2/L): 6.7 - 8.8
- oxygen concentration after 6 min (mg O2/L): 4.0 - 8.4 - Nominal and measured concentrations:
- - concentration tested (mg/L): 1000 (nominal)
- Details on test conditions:
- PREPARATION OF INOCULUM
- Inoculum was washed, brought to concentration of 5 g/L dry substance and aerated during the night. 50 ml were added to a total volume of 250 ml to obtain concentration of 1 g/L dry substance in the test.
- Oxygen concentration during aeration: > 2.5 mg/L
TEST SYSTEM
- Incubation time: 30 min
- Test vessels: Erlenmeyer-vessel (nominal volume 250 ml)
- Test volume: 250 ml
- Synthetic medium: 8 ml/vessel 100-fold concentrated OECD medium
- Duration of the measurement of oxygen consumption: 8 - 10 min
PREPARATION OF TEST SOLUTION
- Concentration of test substance (mg/L): 1000
- Added volume of stock solution (ml/vessel): 125
- Synthetic medium (ml/vessel): 8
- Inoculum (ml/vessel): 50
- Filled up to the total volume with deionized water (ml/vessel): 250 ml - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- EFFECT CONCENTRATIONS
30 min EC20 ca. 2.5 mg/L nominal test mat. respiration rate
30 min EC50 ca. 17 mg/L nominal test mat. respiration rate
30 min EC80 ca. 75 mg/L nominal test mat. respiration rate
Applicant's summary and conclusion
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