Reaction mass of 1,7,7-trimethylbicyclo[2.2.1]hept-2-yl acetate and (1S,2S,4S)-1,7,7-trimethylbicyclo[2.2.1]hept-2-yl acetate

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: No GLP compliance and no data on guidelines

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1980

Materials and methods

Principles of method if other than guideline:

Growth inhibition test

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Exo-1,7,7-trimethylbicyclo[2.2.1]hept-2-yl acetate

EC Number:

204-727-6

EC Name:

Exo-1,7,7-trimethylbicyclo[2.2.1]hept-2-yl acetate

Cas Number:

125-12-2

Molecular formula:

C12H20O2

IUPAC Name:

(1S,2S,4S) 1,7,7-trimethylbicyclo[2.2.1]hept-2-yl acetate

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

Bacillus thuringiensis

Study design

Test type:

not specified

Limit test:

yes

Remarks on exposure duration:

not referenced

Results and discussion

Effect concentrations

Dose descriptor:

EC100

Effect conc.:

<= 40 mg/L

Any other information on results incl. tables

Effect ofterpenes on the growth of 4 Bacillus species:

All the terpenes (including IBA) inhibited Bacillus thuringiensis at levels of the terpene below those found in the foliage of the tree.

Applicant's summary and conclusion

Conclusions:

EC100 <= 40 mg/L

Executive summary:

These studies report the relation-ship between the Douglas fir terpenes and the growth rate, cellular integrity, mitochondrial activity and infectivity of some selected microorganisms.

The Douglas fir terpene alfa-pinene was shown to inhibit the growth of a variety of bacteria and a yeast. Other terpenes of the Douglas fir,including limonene, camphene, and isobornyl acetate, were also inhibitory to Bacillus thuringiensis. All terpenes were inhibitory at concentrations normally present in the fir needle diet of Douglas fir tussock moth larvae. The presence of such terpenes in the diet of these insects was found to strongly influence the infectivity of B. thuringiensi spores for the Douglas fir tussock moth larvae. The terpene alfa-pinene destroyed the cellular integrity and modified mitochondrial activity in certain microorganisms.

The higher concentration of alfa-pinene used in the experiment was assumed to be a reasonable approximation of the effect of total fir needle terpenes on the B. thuringiensis infectivity for Douglas fir tussock moth.

IBA was inhibitory to the growth of Bacillus thuringiensis.