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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1993 - 1994
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well performed OECD study with GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report date:
1994

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
not specified
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
N-(2-methoxy-5-methylphenyl)acetamide
EC Number:
230-157-2
EC Name:
N-(2-methoxy-5-methylphenyl)acetamide
Cas Number:
6962-44-3
Molecular formula:
C10H13NO2
IUPAC Name:
N-(2-methoxy-5-methylphenyl)acetamide
Details on test material:
- Name of test material (as cited in study report):Acetkresidin 34 TTRS
- Substance type: organic
- Physical state: solid

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
Concentrations of the test item: 62.5,125, 250, 500, 1000 mg/L. For each concentration two parallel tests were performed. The required amount of test item was added directly into the test vessel.

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
source: waste water treatment plant Frankfurt-Sindlingen

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Post exposure observation period:
no

Test conditions

Test temperature:
20+-2 °C
pH:
ca. 7.2
Reference substance (positive control):
yes
Remarks:
3,5-DichJorphenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
other: EC20
Effect conc.:
604 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Details on results:
Concentration respiration inhibition of test item
mg/L mean from two tests %
1000 28
500 11.8
250 5.4
125 6.5
62.5 1.1
Results with reference substance (positive control):
Concentration respiration inhibition of 3,5-Dichlorophenol
mg/L %
5 22
20 70.4
30 72
Reported statistics and error estimates:
The results of the respiration rate for each concentration were compiled in a graphics. Out of that the EC50, EC 20 and EC80 values could be determined

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
EC50 for respiration inhibition of Acetkresidin 34 TTRS is > 1000 mg/L.
Executive summary:

Acetkresidin 34 TTRS was tested for its properties to inhibit the respiration rate of activated domestic sludge according to OECD 209.

An EC50 value of > 1000 mg/L was determined.