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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: well performed research study, conducted prior to GLP and OECD guideline implementation

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1975
Report date:
1975

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Company guideline similar to OECD 401
Deviations:
not applicable
GLP compliance:
no
Remarks:
prior to GLP implementation
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
N-(2,5-dimethoxyphenyl)acetamide
EC Number:
222-423-1
EC Name:
N-(2,5-dimethoxyphenyl)acetamide
Cas Number:
3467-59-2
Molecular formula:
C10H13NO3
IUPAC Name:
N-(2,5-dimethoxyphenyl)acetamide
Details on test material:
- Name of test material (as cited in study report): Acetchinonbase

Test animals

Species:
rat
Strain:
Wistar
Sex:
male
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Hoechst AG breeding colony
- Weight at study initiation: mean: 91 g (80 - 118)
- Fasting period before study: 16 hourrs
- Housing: plastic cages
- Diet: Altromin 1324, ad libitum
- Water: tap water, ad libitum

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
other: sesame oil
Doses:
500, 630, 800, 900, 1000, 1600 mg/kg bw
as 10% suspension in sesame oil
No. of animals per sex per dose:
10 male per dose
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: observation: daily; weighing: initially, after 7 days, after 14 days
- Necropsy of survivors and animals found dead performed: yes
- Other examinations performed: clinical signs, body weight
Statistics:
LD50 was determined by a Probit analysis (method according to Lindner and Weber); the confidence interval was calculated according to Cavalli-Sforza

Results and discussion

Effect levelsopen allclose all
Sex:
male
Dose descriptor:
LD50
Effect level:
962 mg/kg bw
Based on:
test mat.
Sex:
female
Remarks on result:
other: no higher sensitivity in a preliminary test
Mortality:
500 mg/kg: 0/10
630 mg/kg: 0/10
800 mg/kg: 0/10
900 mg/kg: 2/10
1000 mg/kg: 7/10
1600 mg/kg: 10/10
Clinical signs:
other: lethally intoxicated animals showed: disturbances of equilibrium, lateral and prone position, reddish eye discharge
Gross pathology:
no abnormal findings
Other findings:
no other findings

Applicant's summary and conclusion

Interpretation of results:
harmful
Remarks:
Migrated information Criteria used for interpretation of results: expert judgment
Conclusions:
LD50: 962 mg/kg bw
Executive summary:

The acute oral toxicity of the test item was determined by application of doses of 500, 630, 800, 900, 1000 and 1600 mg/Kg bw as a 10% suspension in sesame oil to male Wistar-rats. 10 animlas per dose were used. Two animlas died at a dose of 900 mg/kg bw., 7 animals at 1000 mg/kg and ten at 1600 mg/kg bw. LD50 was determined by Probit-Analyse as 962 (918 - 1008) mg/kg bw.