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EC number: 256-435-3 | CAS number: 49701-24-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://www.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1990
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: well performed study according to acknowledged standards but without GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- not specified
- GLP compliance:
- no
Test material
- Reference substance name:
- 4-amino-2,5-dimethoxy-N-methylbenzenesulphonamide
- EC Number:
- 256-435-3
- EC Name:
- 4-amino-2,5-dimethoxy-N-methylbenzenesulphonamide
- Cas Number:
- 49701-24-8
- Molecular formula:
- C9H14N2O4S
- IUPAC Name:
- 4-amino-2,5-dimethoxy-N-methylbenzene-1-sulfonamide
- Details on test material:
- purity:97.8 - 98.0 %
Constituent 1
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- Origin: Domestic sewage treatment plant in Frankfurt / Main - Sindlingen
Content of suspended solids in the sewage sludge suspension : 3.86 g/L
State of acclimatization : not pre-adapted to the test substance or the reference substance
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 19.1 - 20.3 °C
- pH:
- 7.0 - 7.3
- Nominal and measured concentrations:
- nominal: 500 mg/L and 1000 mg/L
- Details on test conditions:
- Incubation vessels: Glasbottles with a diameter of 60 mm and a grounded neck (NS 29), height 170 mm and a total volume of approx. 480 ml. The
bottles contained magnetic stirrer bars and were incubated in a water bath on magnetic stirrers.
Test volume: 200 ml each batch
Temperature control : laboratory thermostat
Aeration rate/test vessel: 45 litres per hour, only during the incubation period
Oxygen measurements: With oxygen electrodes, connected to oximeters
Monitoring: During the measuring periods of 10 minutes without further aeration the oxygen concentrations were measured
Respiration rate: The differences of the O2-concentrations between the measurements at the beginning and the end of the 10-min measuring period without aeration accorded to the corresponding respiration rates
Measurements of the pH: pH-meters - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- other: 2.7 % inhibition
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: respiration inhibition
- Duration:
- 3 h
- Dose descriptor:
- other: 15.3% inhibition
- Effect conc.:
- 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: respiration inhibition
- Results with reference substance (positive control):
- see table below
Any other information on results incl. tables
Table 1: Measurements and calculated inhibition
Controls |
Respiration activity |
Respiration inhibition [%] |
pH value |
Temperature |
Blank 1 |
57.0 |
(-8.8%) |
7.0 |
20.3 |
Blank 2 |
50.3 |
(4.0%) |
7.0 |
19.4 |
Blank 3 |
50.0 |
(4.6%) |
7.2 |
19.8 |
Mean of the blanks |
52.4 |
0% |
|
|
DCP 5 ppm |
39.6 |
24.4% |
7.1 |
20.0 |
DCP 20 ppm |
15.8 |
69.8% |
7.1 |
19.4 |
DCP 30 ppm |
14.0 |
73.8% |
7.3 |
20.2 |
Test batches |
Respiration activity |
Respiration inhibition [%] |
pH value |
Temperature |
1000 mg/L |
57.0 |
-8.8 |
7.1 |
19.9 |
1000 mg/L |
45.0 |
14.1 |
7.0 |
19.8 |
Mean |
51.0 |
2.7 |
|
|
500mg/L |
46.8 |
10.7 |
7.0 |
19.2 |
500mg/L |
42.0 |
19.8 |
7.0 |
19.1 |
Mean |
44.4 |
15.3 |
|
|
DCP:reference controls with 3,5-Dichlorophenol to test the sensitivity of the inoculum
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The results of testing the toxicity of Chinonbase-sulfmethylamide in the “Activated sludge: respiration inhibition test” revealed that:
The EC20, EC50 and EC80 of Chinonbase-sulfmethylamide to activated sludge (domestic sewage treatment plant) are > 1000 mg/L. - Executive summary:
The determination of the acute toxicity of the substance to domestic activated sludge was performed according to OECD guideline 209
in concentrations of 500 mg/L and 1000 mg/L. 3,5 -Dichlorophenol was used as reference substance.
No relevant toxicity to bacteria was found.
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