Benzoic acid, 2-fluoro-5-(methylsulfonyl)-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

May 2013 - July 2013

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: This study has been performed according to OECD and/or EC guidelines and according to GLP principles.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-fluoro-5-methanesulfonylbenzoic acid

EC Number:

689-137-0

Cas Number:

247569-56-8

Molecular formula:

C8 H7 F O4 S

IUPAC Name:

2-fluoro-5-methanesulfonylbenzoic acid

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

BMG sample no. M1302-01397-01
Lot no. 270002C
CAS no. 247569-56-8
Purity 99.86%; treated as 100% pure
Water solubility Soluble

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Results and discussion

Any other information on results incl. tables

Effects on mobility

After 24 and 48 h of exposure, no significant effects (≤10% of immobilization) were observed at the

nominal concentration of 100 mg/l and in the control.

Immobility data are presented in Table 3.

Estimation of EC₅₀and NOEC

The median effect concentration (EC₅₀) with respect to immobilization was estimated to be >100 mg/l

nominal concentration and >68.1 and >67.9 mg/l geometric mean concentrations over a period of 0-24

(GM_0-24h) and 0-48 (GM_0-48h) of exposure, respectively, of 2-Fluoro-5-(methylsulfonyl)benzoic acid.

The no observed effect concentration (NOEC) was ≥100 mg/l nominal concentration and ≥68.1 and ≥67.9

mg/l geometric mean concentrations over a period of 0-24 (GM_0-24h) and 0-48 h (GM_0-48h) of

exposure, respectively, of 2-Fluoro-5- (methylsulfonyl)benzoic acid.

100% of immobilization was not observed.

pH-values and oxygen concentrations

pH-values and oxygen concentrations are presented in Table 4. The temperature during the whole test

period remained constant at 21.0°C (target: 20±2°C). All values were within the acceptable range.

Test concentrations

HPLC analyses of 2-Fluoro-5-(methylsulfonyl)benzoic acid were performed at the beginning, after 24

h, and at the end of the exposure (48 h) (Table 5). The test concentration measured at the

beginning of the exposure was 71.3 mg/l. The measured concentrations after 24 and 48 h of exposure

were 66.2 and 66.8 mg/l, and 68.1 and 67.2 mg/l, respectively. The geometric mean concentrations

over a period of 24 (GM_0-24h) and 48 h (GM_0-48h) were 68.1 mg/l (95% of initial

concentration) and 67.9 mg/l (95% of initial concentration), respectively. The evaluation of the

test was based on the geometric mean (GM) concentrations.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

100% of immobilization was not observed.

Executive summary:

The effective concentrations (EC₁₀, EC₂₀, EC₅₀) of 2-Fluoro-5-(methylsulfonyl)benzoic acid to

Daphnia magna were investigated under static exposure conditions over a period of 48 h.

40 individual Daphnia divided into 2 test vessels were exposed to the test substance.

The only applied nominal test concentration was 100 mg/l. Since 2-Fluoro-5- (methylsulfonyl)benzoic

acid is soluble in water, a 100 mg/l stock solution of the test material was directly prepared in

Daphnia medium.

HPLC analyses of 2-Fluoro-5-(methylsulfonyl)benzoic acid were performed at the beginning, after 24

h, and at the end of the exposure (48 h). The test concentration measured at the beginning of the

exposure was 71.3 mg/l. The measured concentrations after 24 and 48 h of exposure were 66.2 and

66.8 mg/l, and 68.1 and 67.2 mg/l, respectively. The geometric mean concentrations over a period of 24 (GM_0-24h) and 48 h

(GM_0-48h) were 68.1 mg/l (95% of initial concentration) and 67.9 mg/l (95% of initial

concentration), respectively. The evaluation of the test was based on the geometric mean (GM)

concentrations.

After 24 and 48 h of exposure, no significant effects (≤10% of immobilization) were observed at the

nominal concentration of 100 mg/l and in the control.

The median effect concentration (EC50) with respect to immobilization was estimated to be >100 mg/l

nominal concentration and >68.1 and >67.9 mg/l geometric mean concentrations over a period of 0-24

(GM_0-24h) and 0-48 h (GM_0-48h) of exposure, respectively, of 2-Fluoro-5-(methylsulfonyl)benzoic

acid.

The no observed effect concentration (NOEC) was ≥100 mg/l nominal concentration and ≥68.1 and ≥67.9

mg/l geometric mean concentrations over a period of 0-24 (GM_0-24h) and 0-48 h (GM_0-48h) of

exposure, respectively, of 2-Fluoro-5- (methylsulfonyl)benzoic acid.

100% of immobilization was not observed.