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EC number: 617-328-0 | CAS number: 82391-37-5
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: Japanese Industrial Standard test KO102 71 (Acute Toxicity Using Fish)
- Deviations:
- no
- GLP compliance:
- not specified
- Vehicle:
- yes
- Remarks:
- dechlorinated tap water
- Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- TEST ORGANISM
- Common name: Japanese rice fish
- Species: Orizias Latipes
ACCLIMATION
- Acclimation period: one week
- Acclimation conditions (same as test or not): acclimated in flowing dechlorinated tap water
- Feeding frequency during acclimation: no feed was given from 2 days prior to its exposure to the test material. - Test type:
- not specified
- Water media type:
- other: Tap water
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 24.3 – 25.5 ℃
- pH:
- 7.2 – 8.0
- Dissolved oxygen:
- 6.8 – 8.1 mg/L
- Nominal and measured concentrations:
- 0 (control group, dechlorinated tap water) and 0.05, 0.1, 0.5 and 1 %
- Details on test conditions:
- TEST SYSTEM
- Testing liquid: Prepared by diluting the test material with dechlorinated tap water
- No. of organisms per vessel: 7 fish per 5 L
- Aeration: Was carried out during exposure period - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- >= 1 other: %
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- >= 1 other: %
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.5 - < 1 other: %
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- The conditions for managing the test fish during the test period including the acclimation period were satisfactory, and there were no factors that could affect the results of the test.
- Sublethal observations / clinical signs:
Death rate and 96 hour LC50 for each hour in each concentration section.
Death rate Exposure Time Concentration (%) 24 hr 48 hr 96 hr Control section 0 0 0 0.05 0 0 0 0.1 0 0 0 0.5 0 0 0 1 0 0 57 - Validity criteria fulfilled:
- not specified
- Conclusions:
- Under the conditions of this study, the 96 hour LC50 was measured to be between 0.5 and 1 %.
- Executive summary:
The short-term toxicity of the test material to fish was investigated in accordance with the Japanese Industrial Standard test guideline KO102 71 (Acute Toxicity Using Fish).
Japanese Rice fish (Orizias Latipes) were the selected species for this test, which were acclimated under the conditions of flowing dechlorinated tap water for one week. The test material was diluted with dechlorinated tap water and the selected test material concentrations were: 0 (control group, dechlorinated tap water) and 0.05, 0.1, 0.5 and 1 %.
The death rate at 1 % concentration after 96 hours was 57.
Under the conditions of this study, the 96 hour LC50 was measured to be between 0.5 and 1 %.
Reference
Description of key information
Short-term toxicity to fish - Key study (Takahashi, 2003):
Under the conditions of this study, the 96 hour LC50 was measured to be between 0.5 and 1 %.
Key value for chemical safety assessment
Additional information
Short-term toxicity to fish - Key study (Takahashi, 2003):
The short-term toxicity to fish of the test material was assessed according to Japanese Industrial Standard test KO102 71 (Acute Toxicity Using Fish).The study was awarded a reliability score of 2 in accordance with the criteria set forth by Klimisch et al. (1997).
Japanese Rice fish (Orizias Latipes) was the selected species for this test, which were acclimated under the conditions of flowing dechlorinated tap water for one week. The test material was diluted with dechlorinated tap water and the selected test material concentrations were:
0 (control group, dechlorinated tap water) and 0.05, 0.1, 0.5 and 1%.
Under the conditions of this study, the 96 hour LC50 was measured to be between 0.5 and 1 %.
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