MoVNbTe mixed oxide

Long-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

fish early-life stage toxicity

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 26 July 2022 to 5 December 2022

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 210 (Fish, Early-Life Stage Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

(1) Name Mo10V3TeNbO42
CAS name Niobium trivanadium decamolybdenum tellurium dotetraconta oxide
CAS No. 146569-48-4
Molecular formula Mo10V3TeNbO42
Molecular weight 2004.91 g/mol
Structure Crystal structure
(2) Batch No. EX.14402.600
(3) Purity 87.6%
(4) Physicochemical properties
Description Black solid powder
Odor Odourless
Melting point（°C） ≥520
Freezing point（°C）≥520
Boiling point（°C） ≥520
pH value 2.9
Solubility in solvents <10µg/L Nb at 20 °C
(5)Stability Stable in container after opening, in water, in light and in soil.

Analytical monitoring:

yes

Details on sampling:

The solutions of blank control and 1.1 mg/L, 3.7 mg/L, 11 mg/L, 37 mg/L and 114 mg/L test groups were sampled. All test substance groups were collected when freshly prepared and immediately prior to renewal at the start of the study and daily thereafter and at the end of the study.

Vehicle:

no

Details on test solutions:

The transformation/dissolution will be initiated when the metal compounds of Mo10V3TeNbO42 dissolved in aqueous medium, the test solution was prepared according to the references “Guidance Document on Transformation/Dissolution of Metals and Metal Compounds in Aqueous Media (OECD series on testing and assessment number 29). In the solubility test, the concentrations of Mo, V, Te and Nb were gradually dissolved, after the 48 hours stirring periods, the increase rate of dissolving become slow down, so the 48 hours stirring periods is appropriately. The preparation of test solution as follow:
2 days prior to the test – 7 days：1.1 mg, 3.7 mg, 11 mg, 37 mg and 114 mg test substance were weighed into 1L flasks, dissolved with about 300 mL dechlorinated tap water, ultrasonic treatment for 15 minutes, the test suspensions were diluted with dechlorinated tap water to 1000 m L, the nominal loading rates of 1.1 mg/L, 3.7 mg/L, 11 mg/L, 37 mg/L and 114 mg/L test suspensions were obtained. The suspensions were stirred for about 48 hours, and then the test suspensions were filtered with 0.22 μm water phase polyether sulfone (PES) filter membranes, the filtrates of each test group were collected and divided into three replicates as test solutions (200 mL / replicate).
8 days – 31 days: 8.8 mg, 29.6 mg, 88 mg, 296 mg and 912 mg test substance were weighed into flasks, dissolved with about 300 mL dechlorinated tap water, ultrasonic treatment for 15 minutes, the test suspensions were transferred into 10 L vessels and diluted with dechlorinated tap water to 8000 m L, the nominal loading rates of 1.1 mg/L, 3.7 mg/L, 11 mg/L, 37 mg/L and 114 mg/L test suspensions were obtained. The suspensions were stirred for about 48 hours, and the test suspensions were filtered with 0.22 μm water phase PES filter membranes, the filtrates of each test group were collected and divided into three replicates as test solutions (2000 mL / replicate).
Note: the filter membranes were pre-saturated in a small amount of saturated solution for 30 minutes before they used for filtering the test suspension.

Test organisms (species):

other: Gobiocyprus rarus

Details on test organisms:

(1) Species: Rare Minnow (Gobiocypris rarus)
(2) Reason for selection of species: The test species is recommended by “The Guidelines for the Testing of Chemicals, 210 Fish, Early-life Stage Toxicity Test” (Chemical Registration Center of MEP, Second Edition).
(3) Supplier: The first parent fish were purchased from Institute of Hydrobiology, Chinese Academy of Science, they were propagated and sub-cultured in the test laboratory. The brood fish with the batch No. FGr20210831-0902 was bred by test laboratory.
(4) Selection and treatment of embryo: The embryos were obtained by the method of artificial fertilization. The embryos with normal development and good morphology were selected under an anatomical microscope. The normal division embryos are sticky and transparent. However, unfertilized embryos are observed as milky white.
(5) The size of embryo: The embryos exposed not older than 8 hours after fertilized
(6) Number of embryo: 360 embryos.

Test type:

semi-static

Water media type:

freshwater

Remarks:

Dechlorinated tap water was used in this test. Before the test, the test water was dechlorinated by aeration so that the dissolved oxygen concentration reached more than 80% of the air saturation value.

Limit test:

no

Total exposure duration:

34 d

Hardness:

At the start of the test, the total hardness of the blank control and 114 mg/L test substance group were both 105 mg/L (calculated by the CaCO3).

Test temperature:

The temperature of all test vessels measured regularly ranged from 24.7 °C to 25.1°C, and the test environment of water bath was measured continuously ranged from 24.1 °C to 25.3 °C, the temperature was not differ by more than ±1.5 °C between test chambers or between successive days at any time during the test, and the temperature was within the range of 25°C ± 1°C.

pH:

During the test, the pH of the test solution was ranged from 7.65 to 8.40.

Dissolved oxygen:

The dissolved oxygen concentration ranged from 65% to 100% of the air saturation value (ASV) and met the requirement of greater than 60% of ASV.

Salinity:

the salinity of the blank control and the nominal loading rates of 1.1 mg/L, 3.7 mg/L, 11 mg/L, 37 mg/L and 114 mg/L test groups were all 0‰ during the test periods.

Nominal and measured concentrations:

The test substance of Mo10V3TeNbO42 as a kind of metallic oxide, the transformation/dissolution will be initiated when the metal compounds dissolved in aqueous medium, given that the toxicity effect cannot be attributed to a single ion morphology, but to Mo10V3TeNbO42 as a whole, the test results are reported should be based on the nominal loading rates.

Details on test conditions:

(1) Test substance: Mo10V3TeNbO42
(2) Test organism: Embryos of Rare Minnow (within 8 hours after fertilization)
(3) Exposure duration: 34 days
(4) Test concentration: According to the results of preliminary test, five nominal loading rates of 1.1 mg/L, 3.7 mg/L, 11 mg/L, 37 mg/L and 114 mg/L test groups with a geometric factor of 3.2 and one blank control were set in the definitive test (calculated with the purity of 87.6%, the nominal loading rates of the main constituent were 1.0 mg/L, 3.2 mg/L, 10 mg/L, 32 mg/L and 100 mg/L).
(5) Replicate 3 replicates / test group
(6) Number of embryo: 20 embryos / replicate, 60 embryos / test group, 360 embryos in total.
(7) Test water: Dechlorinated tap water
(8) Type of test: Renewal more than 3/4 of volume test solution daily with the way of semi-static test.
(9) Preparation of the test solution : 2 days prior to the test – 7 days: 1.1 mg, 3.7 mg, 11 mg, 37 mg and 114 mg test substance were weighed into 1 L flasks, dissolved with about 300 mL dechlorinated tap water, ultrasonic treatment for 15 minutes, the test suspensions were further diluted with dechlorinated tap water to 1000 mL, the nominal loading rates of 1.1 mg/L, 3.7 mg/L, 11 mg/L, 37 mg/L and 114 mg/L test suspensions were obtained. The suspensions were stirred for about 48 hours, and then the test suspensions were filtered with 0.22 μm water phase polyether sulfone (PES) filter membranes, the filtrates of each test group were collected and divided into three replicates as test solutions (200 mL / replicate).
8 days – 31 days: 8.8 mg, 29.6 mg, 88 mg, 296 mg and 912 mg test substance were weighed into flasks, dissolved with about 300 mL dechlorinated tap water, ultrasonic treatment for 15 minutes, the test suspensions were transferred into 10 L vessels and diluted with dechlorinated tap water to 8000 m L, the nominal loading rates of 1.1 mg/L, 3.7 mg/L, 11 mg/L, 37 mg/L and 114 mg/L test suspensions were obtained. The suspensions were stirred for about 48 hours, and the test suspensions were filtered with 0.22 μm water phase PES filter membranes, the filtrates of each test group were collected and divided into three replicates as test solutions (2000 mL / replicate).
(10) Volume of test solution: 0 day – 9 days: 200 mL / test vessel, 10 days- 34 days: 2000 mL / test vessel.
(11) Temperature of test solutions: The temperature of all test vessels measured regularly ranged from 24.7 °C to 25.1 °C, the temperature of the water bath was measured continuously ranged from 24.1 °C to 25.3 °C.
(12) Photoperiod: 14 hours light / 10 hours darkness
(13) The concentration analysis of Nb, Mo, V and Te in test solutions
The actual concentrations of the Niobium (Nb), Molybdenum (Mo), Vanadium (V) and Tellurium (Te) in the blank control and the nominal loading rates of 1.1 mg/L, 3.7 mg/L, 11 mg/L, 37 mg/L and 114 mg/L at the start of the study and daily thereafter and at the end of the study by Inductively coupled plasma mass spectrometry (ICP-MS).

The test results of the preliminary test of Fish, Short-term Toxicity Test on Embryo and Sac-Fry Stages showed that the mortalities of the nominal loading rates of 1.0 mg/L, 3.2 mg/L, 10 mg/L, 32 mg/L and 100 mg/L were 0% and no abonormal behaviours or characters were observed. Therefore, the definitive test of fish early-life stage was designed with five nominal loading rates of 1.1 mg/L, 3.7 mg/L, 11 mg/L, 37 mg/L and 114 mg/L (the nominal concentration of main constituent calculated by the purity of 87.6% are 1.0 mg/L, 3.2 mg/L, 10 mg/L, 32 mg/L and 100 mg/L, respectively ) and a blank control, 20 embryos for each replicate and three replicates for each test group.

Species: Rare Minnow(Gobiocypris rarus)
Reason for selection of species: The test species is recommended by “The Guidelines for the Testing of Chemicals, 210 Fish, Early-life Stage Toxicity Test” (Chemical Registration Center of MEP, Second Edition).
Supplier: The first parent fish were purchased from Institute of Hydrobiology, Chinese Academy of Science, they were propagated and sub-cultured in the test laboratory. The brood fish with the batch No. FGr20210831-0902 was bred by test laboratory.
Selection and treatment of embryo: The embryos were obtained by the method of artificial fertilization. The embryos with normal development and good morphology were selected under an anatomical microscope. The normal division embryos are sticky and transparent. However, unfertilized embryos are observed as milky white.
The size of embryo: The embryos exposed not older than 8 hours after fertilized
Number of embryo: 360 embryos.

Fodder
Name Brine shrimp
Source The eggs of Brine shrimp were obtained from Shandong Beisen Feed Co., Ltd.
The batch number was 20220711.
The shrimps were obtained after the eggs successfully hatched in artificial sea water.

The test was conducted as semi-static test with renew at least 3/4 volume of test solution daily (As the embryos were obtained and exposed at night of 0 day, to perform the test expediently, the time of the first renewal solution was performed less than 24 hours after the start of the exposure).The embryos with normally developing successfully hatch at 4th day, and all the larvae were fed with lively brine shrimp once time daily at 5th day and thereafter. Before feeding and renewal, about 3/4 volume of test solution was siphoned out and with removing the food residue, subsequently, about 3/4 volume freshly test solution supplemented also by the method of siphoning. The 14# silicone tube was used for siphoning in the whole renewal process. After finishing the renewal test solution, the lively brine shrimp as food was taken into the freshly test solution.

Reference substance (positive control):

not required

Key result

Duration:

34 d

Dose descriptor:

NOELR

Effect conc.:

114 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

number hatched

Key result

Duration:

34 d

Dose descriptor:

LOELR

Effect conc.:

> 114 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

number hatched

Key result

Duration:

34 d

Dose descriptor:

EL10

Effect conc.:

> 37 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

length

Key result

Duration:

34 d

Dose descriptor:

EL10

Effect conc.:

> 37 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

weight

Remarks:

dry weight

Key result

Duration:

34 d

Dose descriptor:

NOELR

Effect conc.:

3.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks:

Surviving rate after successful hatching

Key result

Duration:

34 d

Dose descriptor:

LOELR

Effect conc.:

11 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks:

Surviving rate after successful hatching

Details on results:

-The temperature, pH, dissolved oxygen, hardness and salinity
During the test, the pH of the test solution was ranged from 7.65 to 8.40. The temperature of all test vessels measured regularly ranged from 24.7 °C to 25.1°C, and the test environment of water bath was measured continuously ranged from 24.1 °C to 25.3 °C, the temperature did not differ by more than ±1.5 °C between test chambers or between successive days at any time during the test, and the temperature was within the range of 25°C ± 1°C.
The dissolved oxygen concentration ranged from 65% to 100% of the air saturation value (ASV) and met the requirement of greater than 60% of ASV.
At the start of the test, the total hardness of the blank control and 114 mg/L test substance group were both 105 mg/L (calculated by the CaCO3).
The salinity of the blank control and the nominal loading rates of 1.1 mg/L, 3.7 mg/L, 11 mg/L, 37 mg/L and 114 mg/L test groups were all 0‰ during the test periods.

-Surviving rate after post-hatched:
At the end of test, the surviving rate after post-hatched of blank control was 98%, which met the validity criterion that the surviving rate after post –hatched should be not less than 70%. The surviving rates of 1.1 mg/L, 3.7 mg/L, 11 mg/L, 37 mg/L and 114 mg/L test groups were 97%, 95%, 75%, 35% and 0%, respectively.

Nominal loading rate replicate Total embryos Surviving fish after post-hatched Surviving rate Mean of surviving rate
(individual) （tail） (%) M±SD
blank control
1 20 19 95 98±3
2 20 20 100
3 20 20 100

1.1 mg/L
1 20 19 95 97±3
2 20 20 100
3 20 19 95

3.7 mg/L
1 20 19 95 95±3
2 20 19 95
3 20 19 95

11 mg/L
1 20 18 90 75±22
2 20 17 85
3 20 10 50

37 mg/L
1 20 9 45 35±13
2 20 8 40
3 20 4 20

114 mg/L
1 20 0 0 0±0
2 20 0 0
3 20 0 0

Note：M : mean；SD: standard deviation.

-Hatching rate of embryos / larvae
Results of hatching rate, and cumulative abnormal rate are shown in Table 4.
The blastocyst stage of embryos (about 2 hours-5.25 hours) that not older than 8 hours after fertilized were exposed.
All embryos were completely hatched at 4th day after start of the test, the hatching rate of embryos in blank control was 100%, which had met the validity criterion that the hatched success should be more than 66%. The hatching rates of 1.1 mg/L, 3.7 mg/L, 11 mg/L, 37 mg/L and 114 mg/L test groups were all 100%.

Nominal loading rate replicate Total embryos Number of hatching Hatching rate Mean of hatching rate
(individual) （tail） (%) M±SD
blank control
1 20 20 100 100±0
2 20 20 100
3 20 20 100

1.1 mg/L
1 20 20 100 100±0
2 20 20 100
3 20 20 100

3.7 mg/L
1 20 20 100 100±0
2 20 20 100
3 20 20 100

11 mg/L
1 20 20 100 100±0
2 20 20 100
3 20 20 100

37 mg/L
1 20 20 100 100±0
2 20 20 100
3 20 20 100

114 mg/L
1 20 20 100 100±0
2 20 20 100
3 20 20 100

Note：M : mean；SD: standard deviation.

-Abnormal appearances or behaviours of embryos and larvae or juvenile fish
During the test, no abnormal appearances or behaviours were observed in blank control and 1.1 mg/L, 3.7 mg/L, 11 mg/L and 37 mg/L test groups.
The larvae/juvenile fish of 114 mg/L test group developed spinal curvature and died, there were no other behavioral abnormalities.

-The total lengths of surviving larvae or juvenile fish
At the end of the test, the mean and the standard deviation of total length of surviving larvae or juvenile fish in blank control was 15.78 mm±0.20 mm, coefficient of variation (CV) was 1.2%, which met the validity criterion that the CV should not exceed 20%.
The mean and standard deviation of total length of 1.1 mg/L, 3.7 mg/L, 11 mg/L and 37 mg/L test groups were 15.92 mm±0.22 mm, 16.67 mm±0.36 mm, 16.55 mm±0.65 mm and 17.10 mm±0.54 mm, respectively.

-Wet and dry weights of surviving larvae / juvenile fish
The results of wet weight (blotted dry) of surviving larvae or juvenile fish are shown in Table 8.
At the end of the test, the average and standard deviation of wet weight of surviving larvae / juvenile fish in blank control was 33 mg ± 2mg, the coefficient of variation was 3.2%, which was met the validity criterion that the coefficient of variation should not exceed 20%.
The means and standard deviation of wet weights of 1.1 mg/L, 3.7 mg/L, 11 mg/L and 37 mg/L test groups were 37 mg±2 mg, 40 mg ± 1 mg, 39 mg ± 4 mg and 49 mg ± 5 mg.
The results of dry weight of surviving larvae or juvenile fish are shown in Table 9. At the end of the test, the average and standard deviation of dry weight of surviving larvae or juvenile fish in blank control was 7.2 mg±1.1 mg, the coefficient of variation was 14%, which was met the validity criterion that the coefficient of variation should not exceed 20%. The means and standard deviation of wet weights of 1.1 mg/L, 3.7 mg/L, 11 mg/L and 37 mg/L test groups were 7.5 mg ± 0.43 mg, 8.4 mg ± 0.15 mg, 8.1 mg ± 0.95 mg and 10.5 mg ± 0.49 mg.

-The concentration analysis results of Mo, V, Te and Nb
Element Report No. LOD LOQ The ranges of the time weighed
of anal. method means of test solutions in this test
Mo A22025-S22054-1R 0.157 μg/L 0.524 μg/L 0.829 mg/L - 14.4 mg/L
V A22033-S22054-1R 0.278 μg/L 0.925 μg/L 0.0397 mg/L - 2.18 mg/L
Te A22035-S22090-1R 0.0467 μg/L 0.156 μg/L 0.0521 mg/L - 2.18 mg/L
Nb A22034-S22089-1R 0.0559 μg/L 0.186 μg/L 0.615 μg/L - 4.33 μg/L

Validity criteria fulfilled:

yes

Conclusions:

At the end of the test (34 days), the surviving rates of the blank control and 1.1 mg/L, 3.7 mg/L, 11 mg/L, 37 mg/L and 114 mg/L test groups were 98%, 97%, 95%, 75%, 35% and 0%, respectively.
The test substance of Mo10V3TeNbO42 as a kind of metallic oxide, the transformation/dissolution will be initiated when the metal compounds dissolved in aqueous medium, given that the toxicity effect cannot be attributed to a single ion morphology, but to Mo10V3TeNbO42 as a whole, the test results are reported should be based on the nominal loading rates. The NOEL, LOEL and EL10 of Fish, Early-life Stage Toxicity Test to Rare Minnow (Gobiocypris rarus) embryos are shown as follow:

Indicator EL10 NOEL LOEL
Hatching rate — =114 mg/L >114 mg/L
The surviving rate after success hatching — =3.7 mg/L =11 mg/L
Total length of larvae/juvenile fish >37 mg/L =3.7 mg/L =11 mg/L
Individual wet weight of larvae/juvenile fish (blotted-dry) >37 mg/L =1.1mg/L =3.7 mg/L
Dry weight of larvae/juvenile fish >37 mg/L =37 mg/L > 37 mg/L

Executive summary:

Test conditions

(1) Test substance                                     Mo₁₀V₃TeNbO₄₂

(2) Test organism                                      Embryos of Rare Minnow (within 8 hours after fertilization)

(3) Exposure duration                              34 days

(4) Test concentration                              According to the results of preliminary test (see Annex C), five nominal loading rates of 1.1 mg/L, 3.7 mg/L, 11 mg/L, 37 mg/L and 114 mg/L test groups with a geometric factor of 3.2 and one blank control were set in the definitive test (calculated with the purity of 87.6%, the nominal loading rates of the main constituent were 1.0 mg/L, 3.2 mg/L, 10 mg/L, 32 mg/L and 100 mg/L).

(5) Replicate                                             3 replicates / test group

(6) Number of embryo                             20 embryos / replicate, 60 embryos / test group, 360 embryos in total.

(7) Test water                                            Dechlorinated tap water

(8) Type of test                                          Renewal more than 3/4 of volume test solution daily with the way of semi-static test.

(9) Preparation of the test solution          2 days prior to the test – 7 days: 1.1 mg, 3.7 mg, 11 mg, 37 mg and 114 mg test substance were weighed into 1 L flasks, dissolved with about 300 mL dechlorinated tap water, ultrasonic treatment for 15 minutes, the test suspensions were further diluted with dechlorinated tap water to 1000 mL, the nominal loading rates of 1.1 mg/L, 3.7 mg/L, 11 mg/L, 37 mg/L and 114 mg/L test suspensions were obtained. The suspensions were stirred for about 48 hours, and then the test suspensions were filtered with 0.22 μm water phase polyether sulfone (PES) filter membranes, the filtrates of each test group were collected and divided into three replicates as test solutions (200 mL / replicate).

                                                                   8 days – 31 days: 8.8 mg, 29.6 mg, 88 mg, 296 mg and 912 mg test substance were weighed into flasks, dissolved with about 300 mL dechlorinated tap water, ultrasonic treatment for 15 minutes, the test suspensions were transferred into 10 L vessels and diluted with dechlorinated tap water to 8000 m L, the nominal loading rates of 1.1 mg/L, 3.7 mg/L, 11 mg/L, 37 mg/L and 114 mg/L test suspensions were obtained. The suspensions were stirred for about 48 hours, and the test suspensions were filtered with 0.22 μm water phase PES filter membranes, the filtrates of each test group were collected and divided into three replicates as test solutions (2000 mL / replicate).

(10) Volume of test solution                    0 day – 9 days: 200 mL / test vessel, 10 days- 34 days: 2000 mL / test vessel.

(11) Temperature of test solutions          The temperature of all test vessels measured regularly ranged from 24.7 °C to 25.1 °C, the temperature of the water bath was measured continuously ranged from 24.1 °C to 25.3 °C.

(12) Photoperiod                                      14 hours light / 10 hours darkness

(13) The concentration analysis of Nb, Mo, V and Te in test solutions

The actual concentrations of the Niobium (Nb), Molybdenum (Mo), Vanadium (V) and Tellurium (Te) in the blank control and the nominal loading rates of 1.1 mg/L, 3.7 mg/L, 11 mg/L, 37 mg/L and 114 mg/L at the start of the study and daily thereafter and at the end of the study by Inductively coupled plasma mass spectrometry (ICP-MS).

Test results

(1) The concentration analysis results of Mo, V, Te and Nb

 

Element	The report NO. of analytical method	LOD	LOQ	The ranges of the time weighed means of test solutions in this test
Mo	A22025-S22054-1R	0.157 μg/L	0.524 μg/L	0.829 mg/L - 14.4 mg/L
V	A22033-S22054-1R	0.278 μg/L	0.925 μg/L	0.0397 mg/L - 2.18 mg/L
Te	A22035-S22090-1R	0.0467 μg/L	0.156 μg/L	0.0521 mg/L - 2.18 mg/L
Nb	A22034-S22089-1R	0.0559 μg/L	0.186 μg/L	0.615 μg/L - 4.33 μg/L

(2) Toxicity effects on test organism

At the end of the test, the mortalities of the larvae/ juvenile fish in the blank control and the nominal loading rates of 1.1 mg/L, 3.7 mg/L, 11 mg/L, 37 mg/L and 114 mg/L were 2%，3%，5%，25%，65% and 100%, respectively.

The test substance of Mo₁₀V₃TeNbO₄₂ as a kind of metallic oxide, the transformation/dissolution will be initiated when the metal compounds dissolved in aqueous medium, given that the toxicity effect cannot be attributed to a single ion morphology, but to Mo₁₀V₃TeNbO₄₂ as a whole, the test results are reported should be based on the nominal loading rates. The No Observed Effect loading rate (NOEL) and Lowest Observed Effect loading rate (LOEL) and the 10% effective loading rate of Fish, Early-life Stage Toxicity Test to Rare Minnow (Gobiocypris rarus) embryo are shown as follow:

Indicator	EL10	NOEL	LOEL
Hatching rate	—	=114 mg/L	>114 mg/L
The surviving rate after success hatching	—	=3.7 mg/L	=11 mg/L
Total length of larvae/juvenile fish	>37 mg/L	=3.7 mg/L	=11 mg/L
The individual wet weight of larvae/juvenile fish (blotted-dry)	>37 mg/L	=1.1mg/L	=3.7 mg/L
Dry weight of larvae/juvenile fish	>37 mg/L	=37 mg/L	> 37 mg/L

Description of key information

The effect level is given based on the nominal loading rate of the test item Mo10V3TeNbO42. 
Hatching rate NOEL = 114 mg/L, LOEL > 114 mg/L, Surviving rate after hatching NOEL = 3.7 mg/L, LOEL = 11 mg/L
Length and weight of larvae/juvenile fish EL10 > 37 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

EC10

Remarks:

10% effective loading rate EL10

Effect concentration:

> 37 mg/L