Reaction mass of sodium chloride and potassium chloride and trisodium hexafluoroaluminate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Remarks:

One guideline study (OECD 202) is available for short-term toxicity in invertebr ates. The GLP status is unclear. The 48-h EC50 in Daphnia magna is 156 mg/L in a static test; no analytical monitoring has been performed

Adequacy of study:

key study

Study period:

2003

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other:

Remarks:

GLP status unclear, guideline study, available as unpublised report, minor restrictions, adequate for assessment. However, no analytical monitoring has been performed and the purity of the test substance was not specified.

Justification for type of information:

The read-across can be performed because trisodium hexafluoroaluminate is component parts of ADS 2-5, and is the only component with possible negative effects on the aquatic environment. In view of this fact, we will detail its effect, based on published studies.

Cross-reference

Reason / purpose for cross-reference:

read-across source

Remarks:

In the following is the data from the study available to determine the short-term toxicity of trisodium hexafluoroaluminate on aquatic invertebrates

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Remarks:

One guideline study (OECD 202) is available for short-term toxicity in invertebrates. The GLP status is unclear. The 48-h EC50 in Daphnia magna is 156 mg/L in a static test; no analytical monitoring has been performed (IWL,1998).

Adequacy of study:

weight of evidence

Study period:

1998

Reliability:

2 (reliable with restrictions)

Justification for type of information:

The read-across can be performed because trisodium hexafluoroaluminate is component parts of ADS 2-5, and is the only component with possible negative effects on the aquatic environment. In view of this fact, we will detail its effect, based on published studies.

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

yes temperature variation was one degree Celsius higher than required in 67/548/EEC Annex V C.2

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

Test concentrations were diluted from the stock solution.

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna STRAUS
Organisms were obtained from own culture and they were 6-24 h old.
Food: Scenedesmus subspicatus (green algae)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

Not measured

Test temperature:

20 ± 2°C

pH:

Begin test: 7.7 - 7.9
End test: 8.2 - 8.5

Dissolved oxygen:

Begin test: 8.0 - 9.1 mg/l
End test: 8.7 - 9.3 mg/l

Salinity:

Not applicable

Nominal and measured concentrations:

Nominal: 72, 86, 108, 115, 122, 130 and 144 mg/l

Details on test conditions:

Five instars per test vessel, 50 ml each, four vessels per test concentration were run. Tap water, which was used for cultures, was also used as test water.

Reference substance (positive control):

yes

Remarks:

kaliumdichromat

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 156 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other:

Remarks:

immobilisation

Details on results:

Immobilisation in control vessels (0 mg/l) remained under 10 %. EC50 of 156 mg/l (above observed water solubility) was extrapolated from the test.

Results with reference substance (positive control):

Results with reference substance (positive control):
The EC50 with kaliumdichromat was 1.8 mg/l

Validity criteria fulfilled:

yes

Conclusions:

Trisodium hexafluoroaluminate is toxic to aquatic life with long lasting effects. But in the composition ADS 2-5 Trisodium hexafluoroaluminate is present in a proportion below 10%. Therefore ADS 2-5 may be harmful to aquatic life. Under normal conditions of production, storage, transport and use ADS 2-5 does not affect the environment. Requires only a warning to avoid dispersion in water.

Executive summary:

One guideline study (OECD 202) is available for short-term toxicity in invertebrates. The GLP status is unclear. The 48-h EC50 in Daphnia magna is 156 mg/L in a static test; no analytical monitoring has been performed (IWL, 1998).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

not specified

GLP compliance:

not specified

Test material

Test material information

impurity 1

Reference substance name:

Trisodium hexafluoroaluminate

EC Number:

237-410-6

EC Name:

Trisodium hexafluoroaluminate

Cas Number:

13775-53-6

Molecular formula:

AlF6Na3

IUPAC Name:

trisodium hexafluoroalumanetriuide

Test material form:

solid: particulate/powder

Details on test material:

- Name of test material (as cited in study report): Cryolite
- Substance type: slighty coloured powder
- Analytical purity: 96.9%
- Composition of test material, percentage of components: Na 31%, Al 12.6%, F 53.3%
- Storage condition of test material: room temperature

Specific details on test material used for the study:

Purity not specified

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

Test concentrations were diluted from the stock solution

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna STRAUS
Organisms were obtained from own culture and they were 6-24 h old.
Food: Scenedesmus subspicatus (green algae)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

Not measured

pH:

Begin test: 7.7 - 7.9
End test: 8.2 - 8.5

Dissolved oxygen:

Begin test: 8.0 - 9.1 mg/l
End test: 8.7 - 9.3 mg/l

Salinity:

Not applicable

Nominal and measured concentrations:

Nominal: 72, 86, 108, 115, 122, 130 and 144 mg/l

Details on test conditions:

Five instars per test vessel, 50 ml each, four vessels per test concentration were run. Tap water, w
hich was used for cultures, was also used as test water.

Reference substance (positive control):

yes

Remarks:

kaliumdichromat

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

156 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other:

Remarks:

immobilisation

Details on results:

Immobilisation in control vessels (0 mg/l) remained under 10 %. EC50 of 156 mg/l (above observed water solubility) was extrapolated from the test.

Results with reference substance (positive control):

The EC50 with kaliumdichromat was 1.8 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Trisodium hexafluoroaluminate is toxic to aquatic life with long lasting effects. But in the composition
ADS 2-5 Trisodium hexafluoroaluminate is present in a proportion below 10%. Therefore ADS 2-5 may be harmful to aquatic life. Under normal conditions of production, storage, transport and use ADS 2-5 does not affect the environment. Requires only a warning to avoid dispersion in water.

Executive summary:

One guideline study (OECD 202) is available for short-term toxicity in invertebrates. The GLP status is unclear. The 48-h EC50 in Daphnia magna is 156 mg/L in a static test; no analytical monitoring has been performed