Reaction products of cyclohexanone with buta-1,3-diene and hydrogen peroxide and iron sulphate and methanol, hydrolysed, ammonium salts

Endpoint summary

Description of key information

Short-term toxicity to aquatic invertebrates

The acute toxicity of UB20-LDB (50%) to Daphnia magna was assessed according to OECD Guideline 202 and EU Method C.2. Young daphnids (< 24 hours old) were exposed in a static test to a nominal test item concentration of 100 mg/L, a control and a solvent control (ethylene glycol) (to determine whether any adverse effects of exposure were due to the presence of the solvent) for 48-hours. The number of immobile organisms were after 24 and 48 hours.Analysis of the 100 mg/L test preparations at 0 and 48 hours showed measured test concentrations of 93% and 80% of nominal respectively, thus results were based on the nominal test concentration.The test was considered to be valid given that none of the control daphnids showed immobilisation or other signs of disease or stress and that the oxygen concentration at the end of the test was equal to or greater than 3 mg/L in the control and test vessels. The solvent control group showed no effects in exposed daphnids over the 48-hour exposure period. As such it can be considered that the presence of ethylene glycol in the sample of test item had no effect on the results obtained and that any observed effects would have been as a direct result of exposure to the UB-20 ammonium salts.

  

No immobilisation was shown in daphnids exposed to the 100 mg/L test item concentration over the 48-hour exposure period. The 48-h EC50 value is therefore >100 mg/L.

Toxicity to aquatic algae and cyanobacteria

The toxicity of UB20-LDB (50%) to the freshwater algae, Pseudokirchneriella subcapitata, was assessed according to OECD Guideline 201 and EU Method C.3. Exponentially growing cultures of the algal were exposed to nominal test concentrations of 1.0, 3.2, 10, 32 and 100 mg/L (corresponding to geometric mean measured concentrations of 0.42, 2.2, 9.3, 28 and 88 mg/L) a control and a solvent control (ethylene glycol) (to determine whether any adverse effects of exposure were due to the presence of the solvent) for 72-hours. Samples of the algal populations were taken after 24, 48 and 72-hours for determination of cell densitiesusing a Coulter® Multisizer Particle Counter.

 

Analysis of test preparations at 0 and 72-hours showed measured test concentrations to range from 19% to 84% of nominal indicating that the decline in measured test concentrations was due to both instability under the conditions of the test and adsorption of the test item to the algal cells present. Results were therefore based on geometric mean measured test concentrations. The test was considered to be valid given that the cell concentration of the control cultures increased by a factor of 178 after 72-hours, the mean coefficient of variation for section by section specific growth rate for the control cultures was 14%, and the coefficient of variation for average specific growth rate for the control cultures over the test period (0 to 72 hour) was 2%. The solvent control group containing the same nominal quantity of ethylene glycol as the nominal 100 mg/L test group saw a 4% reduction in growth rate over the duration of the test when compared to the control group. As such it can be considered that the presence of ethylene glycol in the sample of test item had no effect on the results obtained and that any inhibitory effects observed were as a direct result of exposure to the UB-20 ammonium salts.

  

The 72-hour ErC50, NOErC and LOErC values were calculated to be 43 mg/L (95% confidence limits: 30 - 60 mg/L), 9.3 mg/L and 28 mg/L, respectively. The 72-hour EyC50, NOEyC and LOEyC values were calculated to be 17 mg/L (95% confidence limits: 12 - 25 mg/L), 9.3 mg/L and 28 mg/L, respectively.