Reaction mass of Disodium [3-{[4-chloro-2-(hydroxy-kO)-5-methoxyphenyl]diazenyl-kN2}-4-(hydroxy-kO)naphthalene-2,7-disulfonato(4-)](hydroxy)chromate(2-) and Trisodium [3-{[4-chloro-2-(hydroxy-kO)-5-methoxyphenyl]diazenyl-kN2}-4-(hydroxy-kO)naphthalene-2,7-disulfonato(4-)](dihydroxy)chromate(3-)

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: inherent biodegradability

Remarks:

modified Zahn-Wellens-Test

Type of information:

experimental study

Adequacy of study:

key study

Study period:

18 May 1994 to 22 August 1994

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Code number: FAT-20044/B
Batch-Nr.: 99
Appearance: solid
Solubility: 60g/l [in water]
Storage: room temperature
Expiration date: 07/99

Oxygen conditions:

not specified

Inoculum or test system:

sewage, domestic (adaptation not specified)

Details on inoculum:

Sludge of a biological sewage treatment plant. Ara Reinach collected on 17.05.94

Duration of test (contact time):

28 d

Initial conc.:

150 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation:

DOC removal

Reference substance:

diethylene glycol

Parameter:

% degradation (DOC removal)

Value:

0

Sampling time:

28 d

Results with reference substance:

The biodégradation, corrected by the blank control and measured as DOC (mg/l) is: 98.9 % 14th day (average of 2 tests running in parallel).

FAT number: 20044/B

DOC content of substance: 34.5 % (centrifugal)

Biodegradation (28 d): 0 %

Amount of substance (cone.): 435 mg/l

Sludge (cone.): 890 mg/l

Sludge from: Ara Reinach

Analytical determination: Shimadzu TOC Analyzer

Time	Date	Reference DOC (mg/l)	Blind DOC (mg/l)	Subst. 1 DOC (mg/l)	Subst. 2 DOC (mg/l)	Reference Eli. %	Subst. 1 Eli. %	Subst. 2 Eli. %
-1	17.05.94	146.4	-	134.7	141.7
3h	18.05.94	155.3	4.8	137.7	138.4
2.day	20.05.94	147.0	7.3	140.9	141.7	7.2	0.0	0.0
5. day	23.05.94	106.9	9.5	146.3	145.9	35.3	0.0	0.0
7. day	25.05.94	9.8	8.5	146.2	146.8	99.1	0.0	0.0
8. day	26.05.94	9.7	8.5	-	-	99.2	-	-
9. day	27.5.94.	9.3	8.0	145.3	145.2	99.1	0.0	0.0
12. day	30.05.94	9.3	7.6	142.8	143.2	98.9	0.0	0.0
13. day	31.05.94	9.2	7.6	-	-	98.9	-	-
14. day	1.06.94	9.3	7.7	143.3	143.7	98.9	0.0	0.0
16. day	3.06.94	-	7.0	143.4	145.1	-	0.0	0.0
19. day	6.06.94	-	6.7	139.8	142.0	-	0.0	0.0
21. day	8.06.94	-	6.5	142.1	144.7	-	0.0	0.0
23. day	10.06.94	-	6.0	141.8	145.1	-	0.0	0.0
26. day	13.06.94	-	6.4	141.6	145.4	-	0.0	0.0
28. day	15.06.94	-	6.1	141.8	145.4	-	0.0	0.0
					Average 1+2:		0.0
					Adsorption 1+2:		3.5

Validity criteria fulfilled:

yes

Interpretation of results:

not inherently biodegradable

Conclusions:

The biodegradation of FAT 20044/B after 28 days was 0 %.

Executive summary:

The inherent biodegradability of FAT - 20044/B was determined in a Zahn-Wellens-Test according to the OECD Guideline for Testing of Chemicals, No. 302B, Paris 1981 and the EEC-Directive 87/302/EEC, L133 (pp. 99-105), May 1988. The test was performed in compliance with the Good Laboratory Practice (GLP) Regulations of Switzerland. FAT 20044/B and the reference substance Diethylengiycol were tested in concentrations of 150 mg/l DOC. The biodegradation of FAT 20044/B after 28 days was 0 % while that of reference substance after 14 days was 98.9 %.

Reference 2

Endpoint:

biodegradation in water: screening test, other

Remarks:

BOD

Type of information:

experimental study

Adequacy of study:

key study

Study period:

22 June 1994 to 08 August 1994

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.5 (Degradation: Biochemical Oxygen Demand)

Deviations:

yes

Remarks:

Aerate over night after addition of the inoculum

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Code number: FAT 20044/B
Batch-Nr.: 99
Appearance: solid
Solubility: 60g/l [in water]
Storage: room temperature
Expiration date: 07/99

Oxygen conditions:

aerobic

Inoculum or test system:

other: Seeding water of an urban wastewater

Duration of test (contact time):

5 d

Initial conc.:

> 6 - < 800 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Reference substance:

other: Glucose/Glutamic acid

Remarks on result:

not measured/tested

Parameter:

BOD5

Value:

8 mg O2/g test mat.

Results with reference substance:

The BOD5 of Glucose/Glutamic acid solution is 185 mg O2/I.

Validity criteria fulfilled:

yes

Interpretation of results:

other: BOD has been determined

Conclusions:

The BOD5 of FAT - 20044/B is 8 mg O2/g.

Executive summary:

The biological oxygen demand (BOD) study with FAT 20044/B was carried out according to EU method C.5. Based on the study results, the BOD5 of FAT 20044/B corrected by the blank control is 8 mg O2/g while that of reference substance is 185 mg O2/l.

Reference 3

Endpoint:

biodegradation in water: screening test, other

Remarks:

COD

Type of information:

experimental study

Adequacy of study:

key study

Study period:

13 July 1994 to 17 August 1994

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.6 (Degradation: Chemical Oxygen Demand)

Deviations:

yes

Remarks:

Mean of 2 determinations instead of 3

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Code number: FAT 20044/B
Batch-Nr.: 99
Appearance: solid
Solubility: 60g/l [in water]
Storage: room temperature
Expiration date: 07/99

Oxygen conditions:

not specified

Inoculum or test system:

not specified

Duration of test (contact time):

2 h

Initial conc.:

5 mg/L

Parameter followed for biodegradation estimation:

not specified

Reference substance:

other: Potassium hydrogen phthalate solution

Remarks on result:

not measured/tested

Parameter:

COD

Value:

927 mg O2/g test mat.

Results with reference substance:

The COD of Potassium hydrogen phthalate is 203 mg O2/I (Validity: 200 ± 8 mg 02/l)

Experimental conditions

Sample weight: 5.0 mg of the test substance

Temperature: 148 ± 3 °C

Duration: 2 h

Estimation Technique: Titration (Potentiometrie) METROHM Titroprozessor 670 (SAV : 00 111)

Calculation:

The COD of a test substance is calculated from the amount of the unreacted K₂Cr₂O₇(Merck No. 4476), determinated byatitration with (NH₄)Fe(SO₄)₂x 6H₂O (Merck No. 3792) according to

 

( a - b ) x N x 8.0 x 1000 / E = mg O₂/g

 

where

a = consumption (in ml) of 0.12 n (NH₄)Fe(SO₄)₂x 6H₂O for the blank

b = consumption (in ml) of 0.12 n (NH₄)Fe(SO₄)₂x 6H₂O for the test substance

N = Normality of (NH₄)Fe(SO₄)₂x 6H₂O

E = Stock solution (in ml) resp. weight of sample (in mg)

8 = Equivalent of oxygen

Validity criteria fulfilled:

yes

Interpretation of results:

other: COD has determined

Conclusions:

The COD of FAT - 20044/B is 927 mg O2/g.

Executive summary:

The chemical oxygen demand (COD) with FAT 20044/B was carried out according to EU method C.6. Based on the study results, COD of FAT - 20044/B is 927 mg O2/g while that of reference material Potassium hydrogen phthalate is 203 mg O2/l (Validity: 200 ± 8 mg O2/l).

Description of key information

The test substance is non-biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

not biodegradable

Type of water:

freshwater

Additional information

In a key study, the inherent biodegradability of FAT - 20044/B was determined in a Zahn-Wellens – Test according to the OECD Guideline 302B and the EEC-Directive 87/302/EEC, L133 (pp. 99-105), May 1988. The test was performed in compliance with the Good Laboratory Practice (GLP) Regulations of Switzerland. FAT 20044/B and the reference substance Diethylengiycol respectively were tested in concentrations of 150 mg/l DOC. The biodegradation of FAT 20044/B after 28 days was 0 % while that of reference substance after 14 days was 98.9 %. In another key study, biological oxygen demand (BOD) of FAT 20044/B was carried out according to EU method C.5. Based on the study results, the BOD5 of FAT 20044/B corrected by the blank control is 8 mg O2/g while the chemical oxygen demand (COD) with FAT 20044/B is 927 mg O2/g. The ration of BOD/COD is <0.5 so FAT 20044/B is not considered as readily biodegradable.