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Reaction mass of lithium sodium hydrogen 4-amino-6-({5-[(5-chloro-2,6-difluoropyrimidin-4-yl)amino]-2-sulfonatophenyl}diazenyl)-5-hydroxy-3-[(4-{[2-(sulfonatooxy)ethyl]sulfonyl}phenyl)diazenyl]naphthalene-2,7-disulfonate and lithium sodium hydrogen 4-amino-6-({5-[(5-chloro-2,6-difluoropyrimidin-4-yl)amino]-2-sulfonatophenyl}diazenyl)-5-hydroxy-3-{[4-(vinylsulfonyl)phenyl]diazenyl}naphthalene-2,7-disulfonate
EC number: 941-533-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- fish early-life stage toxicity
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 210 (Fish, Early-Life Stage Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0, 1, 3.16 and 10 mg/L
- Days of sampling: -7, 0, 6, 12, 20, 27 and 31
- Sampling method: Not specified
- Sample storage conditions before analysis: Not specified - Vehicle:
- no
- Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- TEST ORGANISM
- Common name: Fathead minnow
- Strain: Rafinesque
- Source: Bio International B.V., Netherlands
- Feeding during test: yes
- Food type: Tetramin fish food
- Amount: 2% of body weight per day
- Frequency: three times daily
ACCLIMATION
- Acclimation period: eight days
- Acclimation conditions (same as test or not): same
- Type and amount of food: Tetramin fish food, 2% of body weight per day
- Feeding frequency during acclimation: three times daily
- Health during acclimation (any mortality observed): <5% - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 32 d
- Hardness:
- 215.3 mg/L CaCO3
- Test temperature:
- 23-27°C
- pH:
- 7.7-8.1
- Dissolved oxygen:
- 6.1-8.7 mg/L
- Nominal and measured concentrations:
- Nominal: 0, 1, 3.16 and 10 mg/L
Measured: 0, 1.17, 3.62 and 11.6 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass vessels, diameter 14 cm x height 7.5 cm and glass aquarium holding test medium
- Size of vessel: glass aquarium 5L
- Aeration: gentle aeration via narrow glass tubes
- Renewal rate of test solution: continuous
- No. of organisms per vessel: 30 fertilised eggs
- No. of vessels per concentration: two
- No. of vessels per control: two
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: freshly prepared reconstituted water
- Culture medium different from test medium: no
- Intervals of water quality measurement: five times during the test
OTHER TEST CONDITIONS
- Photoperiod: 16 h light:8 h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): hatching success, mortality, body abnormalities, type and incidence of abnormalities and length and weight of juvenile fish at the end of the test
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.16 - Key result
- Duration:
- 32 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 11.6 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr. (total fraction)
- Basis for effect:
- number hatched
- Details on results:
- The hatching success was not affected by the test substance exposure in any of the concentrations compared to the control. Further, there were no adverse behavioural and appearance abnormalities in any of the tested concentrations.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the study conditions, the nominal 32 d NOEC of the test substance to the early-life stages of fathead minnow was determined to be >=10 mg/L, equivalent to >=11.6 mg a.i./L.
- Executive summary:
A study was conducted to determine the toxicity of the test substance (in the form of aqueous dispersion of 64% purity) to the early-life stages of fathead minnow (Pimephales promelas) according to OECD Guideline 210, in compliance with GLP. Batches of 60 fertilised eggs, evenly distributed in two replicates were exposed to the substance dispersed in test medium at nominal concentrations of 0, 1, 3.16 and 10 mg/L (equivalent to 0, 1.17, 3.62 and 11.6 mg a.i./L respectively). The test concentrations were prepared through a flow-through device. Observations were made daily on the numbers of fertilised eggs and/or juvenile fish to record the hatching success, behavioural and morphological abnormalities during the exposure period of 32 d. Analytical validation of test concentrations was conducted in regular intervals. There was no significant difference in mortality among the test and control groups during the study and no behavioural abnormalities were noted. Under the study conditions, the nominal 32 d NOEC of the test substance to the early-life stages of fathead minnow was determined to be >=10 mg/L, equivalent to >=11.6 mg a.i./L (Bruns, 2001a).
Reference
Description of key information
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 11.6 mg/L
Additional information
A study was conducted to determine the toxicity of the test substance (in the form of aqueous dispersion of 64% purity) to the early-life stages of fathead minnow (Pimephales promelas) according to OECD guideline 210. Batches of 60 fertilised eggs, evenly distributed in two replicates were exposed to the substance dispersed in test medium at nominal concentrations of 0, 1, 3.16 and 10 mg/L (equivalent to 0, 1.17, 3.62 and 11.6 mg a.i./L respectively). The test concentrations were prepared through a flow-through device. Observations were made daily on the numbers of fertilised eggs and/or juvenile fish to record the hatching success, behavioural and morphological abnormalities during the exposure period of 32 d. Analytical validation of test concentrations was conducted in regular intervals. There was no significant difference in mortality among the test and control groups during the study and no behavioural abnormalities were noted. Under the study conditions, the nominal 32 d NOEC of the test substance to the early-life stages of fathead minnow was determined to be >10 mg/L, equivalent to >11.6 mg a.i./L (Bruns, 2001a).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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