Ozonized olive oil

Ecotoxicological Summary

Additional information

SHORT-TERM TOXICITY TO ACQUATIC INVERTEBRATES:

The acute toxicity of Reaction products of olive oil and ozone (NOVOX) (EC no. 919–979–9) to Daphnia magna was investigated according to the OECD guideline 202 under static exposure conditions over a period of 48 hours.

The test item Reaction products of olive oil and ozone (NOVOX) is a solid and poorly soluble UVCB substance.

The test solution consisted of the Water Soluble Fraction, prepared by addition of a stock solution of test item in hexane to glassware, evaporation of the hexane, addition of test water, stirring for 96 hours and finally filtration.

The single loading rate tested was 100 mg/l. Four replicates for the test concentration as well as for the control, were tested.

The concentrations of Reaction products of olive oil and ozone (NOVOX) in the test media were analysed by DOC analytics at the beginning and after 24 and 48 hours of exposure. The DOC determinations confirmed the very poor solubility of the test item. Conclusions about its stability cannot be drawn, since this type of analytics is non-specific. Therefore, the effective concentrations ECx were assessed based on the loading rate of the test item, which was 100 mg/l.

In the control and at the test concentration of 100 mg/l, no significant number (≤10% immobilisation) of immobilised test organisms was observed during the test period of 48 hours.

The biological test results of Reaction products of olive oil and ozone (NOVOX) to Daphnia magna after 24 and 48 hours of exposure are summarized in the following table based on the loading rate of the test item:

Parameter	Immobilisation (0-24 h)[mg/l]	Immobilisation (0-48 h)[mg/l]
EC50	>100	>100
NOEC	100	100

 

TOXICITY TO ACQUATIC ALGAE AND CYANOBACTERIA:

The growth inhibitory effects of Reaction products of olive oil and ozone (NOVOX) (EC no. 919-979-9) to the green alga Desmodesmus subspicatus were investigated according to

OECD guideline 201 over a period of 72 hours. The test item Reaction products of olive oil and ozone (NOVOX) is a solid and poorly soluble UVCB substance.

The test solution consisted of the Water Soluble Fraction, prepared by addition of a stock solution of test item in hexane to sterile glassware, evaporation of the hexane, addition of sterile test water, stirring for 96 hours and finally filtration.

The single loading rate tested was 100 mg/l. Six parallel test vessels were used for the test item and six for the control.

The concentrations of Reaction products of olive oil and ozone (NOVOX) in the test media were analysed by DOC analytics at the beginning and after 24, 48 and 72 hours of exposure. The DOC determinations confirmed the very poor solubility of the test item. Conclusions about its stability cannot be drawn, since this type of analytics is non-specific. Therefore, the effective concentrations ECx were assessed based on the loading rate of the test item, which was 100 mg/l.

With respect to the endpoint average specific growth rate no significant effects were observed at 100 mg/l loading rate compared to the control.

With respect to the endpoint yield no significant effects were observed at 100 mg/l loading rate compared to the control.

The results of the average specific growth rate and yield inhibition of Reaction products of olive oil and ozone (NOVOX) to the green alga Desmodesmus subspicatus are summarized in the following table based on the loading rate of the test item:

Parameter (0-72 h)	Growth rate [mg/l]	Yield [mg/l]
EC50	>100	>100
NOEC	100	100

 

Conclusion on classification

In conclusion, Reaction products of olive oil and ozone (NOVOX) (EC no. 919–979–9) had no acute toxic effects on Daphnia magna up to its solubility limit in test water under the conditions of the test AND had no significant inhibitory effects on the green algae Desmodesmus  ubspicatus up to its solubility limit in test water under the conditions of the test.