Cuprate(4-), [C-(aminosulfonyl)-C-[[[2-[[4-chloro-6-[(2,5-disulfophenyl)amino]-1,3,5-triazin-2-yl]amino]ethyl]amino]sulfonyl]-29H,31H-phthalocyanine-C,C-disulfonato(6-)-N29,N30,N31,N32]-, sodium salts

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

24 March 1997 to 26 March 1997.

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Deviations:

not specified

Principles of method if other than guideline:

The procedure employed is that outlined in the draft "HPLC-screening method for the determination of the adsorption-coefficient on soil-comparison of different stationary phases", W Kordel, J Stutte and G Kotthoff, Fraunhofer - lnstitut fur Umweltchemie und Okotoxikologie.

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Media:

soil

Specific details on test material used for the study:

No further details specified in the study report.

Radiolabelling:

no

Test temperature:

30 °C

Details on study design: HPLC method:

Dead time
The dead time was determined by measuring the retention time of a 292 mg/l solution of formamide (purity 99.5%*) in methanol.

The HPLC parameters were as follows:
Column: Hypersil CN – BDS 5μ (250 x 4.6 mm id)
Column temperature: 30 °C
Mobile phase: methanol:water (50:50) *
Flow rate: 1 ml/min
Injection volume: 25 μl
Wavelengths: reference standards and dead time - 210 nm; sample - 343 nm
* pH - 7.40.

Calculation
The capacity factor (k) is calculated using equation:

k = (tR – t0) / t0

where:
tR = retention time of the substance (mins)
t0 = dead time (mins)

Key result

Type:

Koc

Value:

< 21.4 dimensionless

pH:

7.4

Temp.:

30 °C

Matrix:

Soil

Remarks on result:

other: % Org. carbon not specified

Key result

Type:

log Koc

Value:

< 1.33 dimensionless

pH:

7.4

Temp.:

30 °C

Matrix:

Soil

Remarks on result:

other: % Org. carbon not specified in the study report.

Details on results (HPLC method):

Under the conditions of the test, the test material showed negligible retention on the HPLC column. The log10 Koc was deduced by direct comparison of the sample retention time with that of the reference standard having the lowest log10 K0c value.

 Dead time

The retention time determined for formamide in shown below:

 

Injection	Retention time (mins)
1 2	3.418 3.418
Mean	3.418

 

Calibration

The retention times obtained for the calibration standards are shown below:

 

Standard	Retention time (mins)
	Injection 1	Injection 2	Mean
N-methylbenzamide Benzamide 2-nitrobenzamide 2-chlorobenzamide Simazine	3.867 3.769 3.779 3.794 4.419	3.869 3.768 3.779 3.798 4.418	3.868 3.769 3.779 3.796 4.419

 

SOIL ADSORPTION COEFFICIENT

The capacity factors (k) for the reference standards were determined from the mean retention times. The capacity factors are shown below together with the literature values for log10 Koc:

 

Standard	Capacity factor k	Log10 k	Log10 Koc
N-methylbenzamide Benzamide 2-nitrobenzamide 2-chlorobenzamide Simazine	0.132 0.103 0.106 0.111 0.293	-0.881 -0.989 -0.976 -0.956 -0.534	1.33 1.34 1.44 1.47 1.77

 

Sample

The retention times of the test material are shown below:

 

Injection	Retention time (mins)	Capacity factor k	Log10 k	Log10 Koc	Mean Log10 Koc	Koc
1 2	1.932 1.935	-0.435 -0.434	N/A* N/A*	<1.33ꝉ <1.33ꝉ	<1.33	<21.4

Log10 Koc: <1.33

Koc: <21.4

* logarithmic values do not exist for negative numbers.

ꝉ determined with reference to the log10 Koc and retention time data shown in tables above.

Validity criteria fulfilled:

not specified

Conclusions:

The soil adsorption coefficient has been determined to be less than 21.4, log10 Koc < 1.33, using an HPLC screening method.

Executive summary:

DETERMINATION OF SOIL ADSORPTION COEFFICIENT

 

The soil adsorption coefficient of JPR Blue 100 has been determined to be less than 21.4, log10 Koc < 1.33, using an HPLC screening method.

Description of key information

The soil adsorption coefficient has been determined to be less than 21.4, log10 Koc < 1.33.

Key value for chemical safety assessment

Koc at 20 °C:

21.4

Additional information

The soil adsorption coefficient has been determined to be less than 21.4, log10 Koc < 1.33, using an HPLC screening method.

[LogKoc: 1.33]