Ethanamine, N-ethyl-, reaction products with polyethylene-polypropylene glycol ether with trimethylolpropane (3:1) acrylate (>1 <6.5 mol EO and >1 < 6.5 mol PO)

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

1

Reference substance name:

Ethanamine, N-ethyl-, reaction products with polyethylene-polypropylene glycol ether with trimethylolpropane (3:1) acrylate (>1 <6.5 mol EO and >1 < 6.5 mol PO)

EC Number:

605-659-3

Cas Number:

173046-61-2

Molecular formula:

Mixture of C6H11O3-[C3H6O]p-[C2H4O]q-[C3H3O]r-C7H14ON and C6H11O3-[C3H6O]p-[C2H4O]q-[C3H3O]s With: 0

IUPAC Name:

Ethanamine, N-ethyl-, reaction products with polyethylene-polypropylene glycol ether with trimethylolpropane (3:1) acrylate (>1 <6.5 mol EO and >1 < 6.5 mol PO)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
Name of test substance: Laromer LR 8889 X
Batch No.: 1/92
Degree of purity: 97.81%

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
Animal species: Zebra fish (Brachydanio rerio HAM. and BUCH.)
Conslgnment dated: Nov. 23, 1993
Supplier: West Aquarium GmbH
Bad Lauterberg, FRG
Breeder: cf. supplier
Body length: 3.29 cm (range: 2.8 - 3.6)
Body welght: 0.305 g (range: 0.16 - 0.45)
Corpulence factor of the batch*: 0.86 (determlned Jan. 26, 1994)
Age: about 5 months

Hou slng:
The fish were kept in a flow-through tank in tap water, not chlorinated,
passed through an active carbon filter and aerated with oi1-
free air.
Photoperlod :16 hours llght and 8 hours dark
Total hardness :about 2.5 mmol/l (- about 250 mg/l CaC03)
Acid capaclty about 5.5 mmol/l
Oxygen content :> 60% of maximum saturation
pH .about 8.0


ACCLIMATION
- Conditions equivalent to housing
- Health during acclimation (any mortality observed): 0% mortality during the last 14 days prior to test initiation

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

Total hardness: about 2.5 mM (about 250 mg/L CaCO3)
Acid capacity: about 5.5 mM

Test temperature:

22 - 23 °C

pH:

8.0 - 8.6

Dissolved oxygen:

5.6 - 8.3 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: all-glass aquaria, 30 x 22 x 24 cm, 10 L fill volume (water depth about 17 cm)
- Aeration: slight
- No. of organisms per vessel: 10
- Biomass loading rate: 0.3 g/L


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Municipal water of the city of Frankenthal, not chlorinated, char coal filtered
- Ca/Mg ratio: 9:1
- Intervals of water quality measurement: after 1, 24, 48, 72 and 96 hours


OTHER TEST CONDITIONS
- Photoperiod: 16 hours light : 8 hours dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality and symptoms


TEST CONCENTRATIONS
- Spacing factor for test concentrations: approximately 2.2
- Range finding study
- Test concentrations: 0.215, 0.464, 1.00, 2.15. 4.64 and 10.0 m/gl
- Results used to determine the conditions for the definitive study: yes

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

2.2 - 4.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes