2-Thiazolamine, 4-(2-naphthalenyl)-

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Study test phase 2012-05-23 to 2012-06-27. Study report date 2012-08-29.

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Report on substance meets the criteria for classification as reliable without restriction to Klimisch et al (1997).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2012

Report date:

2012

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

4-(naphthalen-2-yl)-1,3-thiazol-2-amine

EC Number:

606-740-6

Cas Number:

21331-43-1

Molecular formula:

C13H10N2S

IUPAC Name:

4-(naphthalen-2-yl)-1,3-thiazol-2-amine

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge: Activated sludge from Totnes Sewage Works, Totnes, Devon, UK.
- Storage conditions: Aerated at room temperature and pH 7.0 +/-1.0
- Preparation of inoculum for exposure: Seven days prior to exposure the activated sludge was centrifuged, washed and resuspended in the mineral medium. The sludge was diluted in medium, added to test bottles and stirred until required for use.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

32 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Test temperature: 22+/-0 deg. C
- pH: 7.4 +/-0.2
- pH adjusted: adjusted as necessary

TEST SYSTEM
- Measuring equipment: Oxitop respirometer
- Test performed in open system: no
- Details of trap for CO2: KOH placed in a sealed cup in the neck of the test bottles

SAMPLING
- Sample storage before analysis: ambient temperature

CONTROL AND BLANK SYSTEM
- Inoculum blank: Inoculum blanks containing no test or reference substance were used to deomnstrate there was no other carbon source in the medium

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (O2 consumption)

Value:

< 5

Sampling time:

5 d

% Degradation 2

Parameter:

% degradation (O2 consumption)

Value:

< 5

Sampling time:

10 d

% Degradation 3

Parameter:

% degradation (O2 consumption)

Value:

< 5

Sampling time:

15 d

% Degradation 4

Parameter:

% degradation (O2 consumption)

Value:

< 5

Sampling time:

20 d

% Degradation 5

Parameter:

% degradation (O2 consumption)

Value:

< 5

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

The reference substance attained a maximum mean level of biodegradation (based on BOD:ThOD ratio) of 67%. More than 60% biodegradation was acheived within the 10 day window.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

It is concluded that in this study the test substance failed to meet the criteria for ready biodegradability.

Executive summary:

Introduction

This study was undertaken to determine the Ready Biodegradability of the test substance by measurement of biochemical oxygen demand (BOD) in a manometric respirometry test.

Results

The test material attained a maximum mean level of biodegradation of <5% during this study.

Conclusion

It is concluded that in this study the test substance failed to meet the criteria for ready biodegradability.