Reaction mass of tetrahydroxysilane and choline chloride and calcium chloride and water

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Reaction mass of tetrahydroxysilane and choline chloride and calcium chloride and water

IUPAC Name:

Reaction mass of tetrahydroxysilane and choline chloride and calcium chloride and water

Test material form:

liquid

Specific details on test material used for the study:

Batch 17I13, clear pale yellow liquid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Samples were taken for analysis at 0 and 48 hours and provided to the Sponsor for analysis. of Si by GFAAS

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

The test was carried out using first instar Daphnia magna derived from in-house laboratory cultures.
Adult daphnids were maintained in 150 mL glass beakers containing 100 mL Elendt M7 medium in a temperature controlled room maintaining the water temperature at 18 to 22 °C. The lighting cycle was controlled to give a 16 hours light and 8 hours darkness cycle with 20 minute dawn and dusk transition periods. Each culture was fed daily with a mixture of algal suspension (Desmodesmus subspicatus) and Tetramin flake food suspension. Culture conditions ensured that reproduction was by parthenogenesis. Gravid adults were isolated the day before initiation of the test, such that the young daphnids produced overnight were less than 24 hours old. These young were removed from the cultures and used for testing. The diet and diluent water are considered not to contain any contaminant that would affect the integrity or outcome of the study.

Study design

Test type:

static

Water media type:

freshwater

Remarks:

Reconstituted Water – ISO Medium

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

The reconstituted water had an approximate theoretical total hardness of 250 mg/L as CaCO3.

Test temperature:

21°C

pH:

7.7-7.9

Dissolved oxygen:

8.8-8.9 mg O2/L

Nominal and measured concentrations:

nominal: 100 mg/L
measured: 98 (0h) and 93 (48h) mg/L

Details on test conditions:

In the definitive test, 180 mL plastic vessels containing approximately 100 mL of test
preparation were used. At the start of the test five daphnids were placed in each test and
control vessel at random, in the test preparations. Four replicate test and control vessels were
prepared. The test vessels were then covered to reduce evaporation and maintained in a
temperature controlled room maintaining the water temperature at 18 to 22 °C with a
maximum deviation of ±1 °C with a photoperiod of 16 hours light (between 200 and
1200 Lux) and 8 hours darkness with 20 minute dawn and dusk transition periods. The
daphnids were not individually identified, received no food during exposure and the test
vessels were not aerated.
The control group was maintained under identical conditions but not exposed to the test item.
The test preparations were not renewed during the exposure period.

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The test was considered to be valid given that no more than 10% of the control daphnids showed immobilization or other signs of disease or stress and that the oxygen concentration at the end of the test was equal to or greater than 3 mg/L in the control and test vessels.

Results with reference substance (positive control):

The results from the positive control with potassium dichromate resulted in an 48-h EC50 of 0.54 mg/L and were within the normal range for this reference item.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity of the test item to the freshwater invertebrate Daphnia magna has been investigated and gave a 48-Hour EC50 value of greater than 100 mg/L. The NOEC was equal to or greater than 100 mg/L.