Registration Dossier
Registration Dossier
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EC number: 219-673-9 | CAS number: 2495-35-4
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 20 Nov 2017 - 03 Mar 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Benzyl acrylate
- EC Number:
- 219-673-9
- EC Name:
- Benzyl acrylate
- Cas Number:
- 2495-35-4
- Molecular formula:
- C10H10O2
- IUPAC Name:
- benzyl acrylate
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 1B182601
- Expiration date of the lot/batch: 06 Mar 2018
- Purity: 99.86%
- Physical state/Appearance: clear colourless liquid
- Storage Conditions: room temperature in the dark
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 1.0, 1.8, 3.2, 5.6 and 10 mg/L
- Sample storage conditions before analysis: All samples were stored frozen prior to analysis
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Age of parental stock: less than 24 hours old
Study design
- Test type:
- semi-static
- Water media type:
- other: Elendt M7 Medium
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 20 - 21 °C
- pH:
- 0 hours: 81 - 8.2
24 hours: 7.9 - 8.0
48 hours: 7.8 - 7.9 - Dissolved oxygen:
- 0 hours: 8.9 - 9.0 mg O2/L
24 hours: 8.6 - 8.8 mg O2/L
48 hours: 8.5 - 9.0 mg O2/L - Nominal and measured concentrations:
- Nominal Test Concentration (mg/L): 1.0, 1.8, 3.2, 5.6 and 10.0
Geometric Mean Measured Test Concentration (mg/L): 0.57, 1.1, 2.0, 3.9 and 7.4 - Details on test conditions:
- TEST SYSTEM
- Test vessel: 150 mL glass jars
- Type: closed
- Fill volume: 100 mL
- Type of flow-through: semi-static
- Renewal rate of test solution: once after 24 hours
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water (Elendt M7 medium)
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours darkness for a period of 48 hours with 20 minute dawn and dusk transition periods
VEHICLE CONTROL PERFORMED: no
RANGE-FINDING STUDY
- Test concentrations: 0.1, 1.0, 10.0 and 100 mg/L - Reference substance (positive control):
- yes
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 4 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- other: 95% Confidence Limits
- Effect conc.:
- >= 3.4 - <= 4.8 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 48 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- ca. 4 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Reported statistics and error estimates:
- The EC50 values and associated confidence limits at 48 hours and the slope of the response curve and its standard error were calculated by Logit analysis using Linear Maximum-Likelihood regression. The LOEC and the NOEC at 24 and 48 hours were calculated using the Fisher’s Exact Binomial Test with Bonferroni correction.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Time point: 48 hours
EC50 (mg/L): 4.0
95% Confidence Limits (mg/L): 3.4 - 4.8
NOEC (mg/L) : 48
LOEC (mg/L): 4.0
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