Zinc bis(N-ethyl-N-phenyldithiocarbamate)

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Deviations:

yes

Remarks:

The method was modified according to EG ring test: Assessment of Biodegradability of Chemicals in Water by manometric Respirometry (DG X 1 283/82 Rev. 5)

Principles of method if other than guideline:

A measured volume of mineral medium, containing a known concentration of the test chemical (dissolved or suspended), is stirred in a closed flask with adapted activated sludge at a constant temperature (20 +/-1°C) for up to 28 days. The amount of oxygen taken up by the test chemical (corrected for uptake by blank inoculum, run in parallel) is expressed as a percentage of theoretical oxygen demand (ThOD) or chemical oxygen demand (COD).

GLP compliance:

yes

Remarks:

This test was performed according to OECD guideline for GLP, with the exception of determination of stability, homogeneity and concentration of the substance in testing medium. Analysis was performed using nominal concentrations.

Specific details on test material used for the study:

Purity: 100%

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

Origin: 10L from laboratory plant (Wupperverband)
Sampling: 10.01.1990
Pre-treatment: no
Concentration of inoculum: 30 mg/l (dry substance)

Duration of test (contact time):

28 d

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Reference substance:

aniline

Key result

Parameter:

other: Biodegradation in %

Value:

0

Sampling time:

28 d

Remarks on result:

other: Not readily biodegradable

Parameter:

ThOD

Value:

1 921 mg O2/g test mat.

Results with reference substance:

The reference substance aniline showed a degradation of 69% using the same inoculum.

Validity criteria fulfilled:

yes

Remarks:

Determined result is valid, as the reference substance aniline showed a degradation of 69% using same inoculum. Inspection of test, raw data and report were controlled by the quality assurance at times.

Interpretation of results:

not readily biodegradable

Conclusions:

Vulkacit P extra N shows a biodegradation of 0% and is therefore not readily biodegradable.

Executive summary:

Vulkacit P extra N is analysed under GLP conditions according to MITI-Test (OECD guideline 301 C) in closed bottle under aerobic conditions. Measured theoretical oxygen demand of 100 mg/L test substance is 1921 mg/g. Determined biodegradation of Vulkacit P extra N is 0% after 28 days, it is therefore not readily biodegradable.

Description of key information

Vulkacit P extra N is analysed under GLP conditions according to MITI-Test (OECD guideline 301 C) in closed bottle under aerobic conditions. Measured theoretical oxygen demand of 100 mg/L test substance is 1921 mg/g. Determined biodegradation of Vulkacit P extra N is 0% after 28 days, it is therefore not readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed