3-amino-2,4,6-triiodobenzoic acid

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

April 1999 to Mary 1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: EC guideline no. 92/69/EEC (2)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-amino-2,4,6-triiodobenzoic acid

EC Number:

221-493-0

EC Name:

3-amino-2,4,6-triiodobenzoic acid

Cas Number:

3119-15-1

Molecular formula:

C7H4I3NO2

IUPAC Name:

3-amino-2,4,6-triiodobenzoic acid

Test material form:

solid: bulk

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Schering AG, 37055008

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

The organic carbon concentration of the test solutions were analyzed with a TOC-analyzer at the start of the exposure and 48 hours thereafter. The substance concentration was calculated on the basis of the molecular formula (C7H4IcNO2 with molecular weight of 514.828). The mean concentrations are in the following table.

Test solutions

Vehicle:

yes

Remarks:

tap water

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: Schering AG, Experimental Toxicology, Berlin, Germany
- Age: juveniles (for tap water control) 0-24 hours
- Feeding during test: no

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

Dilution water characteristic: 252 mg/L

Test temperature:

20.7 - 22.0 degrees Celcius

Dissolved oxygen:

8.2 - 8.5 mg/L

Conductivity:

Dilution water characteristic: 715 µS/cm

Nominal and measured concentrations:

For the preparation of the test solution, a solution with nominal loading of 111 mg/L was stirred for 15 minutes. The solution obtained by this procedure was used for the limit test. For the definitive test this solution was further diluted 1:1, 1:2, and 1:4 in order to obtain the final concentrations of 12.5, 25, 50, and 100 mg/L. The mean concentration of the test soltuion calculated based on TOC-analysis was within 86-105% of the nominal which was considered to be in good agreement.

Details on test conditions:

TEST SYSTEM
- Test vessel: not described
- Type (delete if not applicable): not reported
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4

VEHICLE CONTROL PERFORMED: yes

RANGE-FINDING STUDY
- Test concentrations: 100 mg/L (limit test)
- Results used to determine the conditions for the definitive study: no mortality

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

Nominal concentration of ZK 2218 (mg/L)	Concentration of ZK 2218 obtained by TOC-analysis (mg/L)	24 hours	48 hours
0 (Limit and definitive test) control (tap water)	----	0	0
100 (limit test)	104	0	0
12.5	11.00	1	3
25	21.00	0	1
50	47.00	0	0
100	105.00	0	0

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The results of the first limit test were not reproducible and therefore considered as an artefact. The repeat limit test and the definitive test showed consistently the absence of a substance related efffect. Therefore, it was concluded that the EC50(48h) for the test substance to Daphnia is greater than 100 mg/L (nominal).

Executive summary:

Daphnia magna were used to determine the toxicity of the test substance, TK 2218 using OECD 202 and EU Method EC guideline no. 92/69/EEC (2). Two limit tests and a definitive test were conducted. The results of the first limit test were not reproducible and therefore considered as an artefact. The repeat limit test and the definitive test showed consistently the absence of a substance related efffect. Therefore, it was concluded that the EC50(48h) for the test substance to Daphnia is greater than 100 mg/L (nominal).