Dizinc pyrophosphate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

13. Now -17. Nov 2003

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

No GLP, no analytical test concentration verification.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

not specified

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test was conducted using the water soluble fraction of the substance. The test solutions was prepared by adding the required amount of test substance to the test medium and shaking the mixture for 24 hours. Subsequently the mixture was filtered (membrane filtration).

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Source: reared at the testing facility, originally obtained from Umweltbundesamt, Germany in 2003
- Age of parental stock (mean and range, SD): 0-24 h

ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not): temperature 20 ± 1 °C, 16:8 h day night regime
- Type and amount of food: green algae and Tetra Animin ®

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

20 ± 1°C

pH:

Test start: 6.6 - 7.7
Test end: 7.7 - 8.0

Dissolved oxygen:

7.9 - 9.0 mg/L

Nominal and measured concentrations:

Nominal test substance concentrations: control, 5.6, 10, 18, 32, 56, 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 50 ml glass beakers
- Fill volume: 20 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dilution water acc. to ISO 6341


OTHER TEST CONDITIONS
- Adjustment of pH: no

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 48 hours

RANGE-FINDING STUDY
- Test concentrations: control, 10, 100, 1000 mg/L
- Results used to determine the conditions for the definitive study: 20% immobility at 10 mg/L and 80% immobility at 100 mg/L

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

26 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

other: NOEL

Effect conc.:

< 5.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

EC50 (24 h): 2.1 mg/L

Immobile Daphnids after 48 hours of exposure

Test concentrations [mg/L]	Immobile D. magna [%]
0	5
100	90
56	65
32	45
18	40
10	30
5.6	10

Validity criteria fulfilled:

yes

Description of key information

EC(L)50 (48 h): 26 mg/L (nominal)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

26 mg/L

Additional information

The acute toxicity of Dizinc pyrophosphate to aquatic invertebrates was determined in a study following OECD guideline 202. Daphnids were exposed for 48 hours to nominal test substance concentrations of 5.6, 10, 18, 32, 56 and 100 mg/L. The test solutions were prepared separately by adding the required amount of test item to the test medium and subsequent shaking of the mixture for 24 hours. The water soluble fraction was separated by filtering (membrane filtration). Based on the mobility an EC(L)50 (48 h) of 26 mg/L was determined.