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EC number: 259-201-9 | CAS number: 54528-00-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 15 Feb. 2010 to 10 Jun. 2010
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- yes
- Principles of method if other than guideline:
- Deviations :
• The temperature for the main and reference study lay in a range of 21 — 25 °C and therefore higher than stated in the guideline. Because the controls showed a normal exponential growth, this can be stated as uncritical.
• In OECD Guideline 201 and EU Method C.3, different compositions of stock solution II are stated. The study was conducted in accordance with OECD Guideline 201 , therefore, the composition of stock solution II must be stated as deviation from EU Method C.3. - GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Hexahydrocyclopenta[c]pyrrol-2(1H)-amine
- EC Number:
- 259-201-9
- EC Name:
- Hexahydrocyclopenta[c]pyrrol-2(1H)-amine
- Cas Number:
- 54528-00-6
- Molecular formula:
- C7H14N2
- IUPAC Name:
- octahydrocyclopenta[c]pyrrol-2-amine
- Test material form:
- liquid
- Remarks:
- Colourless
Constituent 1
- Specific details on test material used for the study:
- A solution stock solution containing 125 mg test item /L (nominal concentration) in deionised water was prepared. The concentrations to be tested were prepared by diluting of this stock solution with deionised water.
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Stock Solution I:
NH4Cl 1500 mg
MgC12*6H20 1200 mg
CaCl2*2H20 1800 mg
MgS04*7H20 1500 mg
KH2P04 160 mg
H2O deionised ad 1000 mL
Stock Solution II:
FeCl3*6H20 64 mg
NaEDTA*2H20 100 mg
H2O deionised ad 1000 mL
Stock Solution III:
H3B03 185 mg
MnCl2*4H20 415 mg
ZnCl2 3 mg
CoCl2*6H20 1.5 mg
CuCl2*2H20 0.01 mg
Na2MoO4*2H20 7 mg
H2O deionised ad 1000 mL
Stock Solution IV:
NaHCO3 50 g
H2O deionised ad 1000 mL
Pre-culture-medium
Stock Solution I 10.0mL
Stock Solution II 1.0mL
Stock Solution III 1.0mL
Stock Solution IV 1.0mL
H2O deionised ad 1000mL
Nutrient-medium (10-fold concentrated)
Stock Solution I 100mL
Stock Solution II 10mL
Stock Solution III 10mL
Stock Solution IV 10mL
H2O deionised ad 1000mL
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- The culture of Desmodesmus subspicatus was obtained in Dec. 2009 by MBM Sciencebridge GmbH (Institut für Pflanzenpohysiologie of Universität Göttingen). The algae are kept as stock culture on solid agar at 8 C. From an aliquot of this stock culture the pre-culture was prepared.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- Temperature range was 21-25°C.
- pH:
- The pH values range was 7.3 to 7.7.
- Dissolved oxygen:
- Not specified
- Salinity:
- Not specified
- Conductivity:
- Not specified
- Nominal and measured concentrations:
- Nominal : 0 ; 1 ; 10 & 100 mg/L
- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate K2Cr207 (CAS No. 7778-50-9) was used as positive control.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: AUC or Area Under Curve according to EU Method C.3
- Remarks:
- means the integral of the biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield (according to OECD Guideline 201)
- Remarks:
- It is defined as the biomass at the end of the exposure period minus the biomass at the start of the exposure period.
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: AUC or Area Under Curve according to EU Method C.3
- Remarks:
- means the integral of the biomass
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield (according to OECD Guideline 201)
- Remarks:
- It is defined as the biomass at the end of the exposure period minus the biomass at the start of the exposure period.
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 - < 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: AUC or Area Under Curve according to EU Method C.3
- Remarks:
- means the integral of the biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- < 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield (according to OECD Guideline 201)
- Remarks:
- It is defined as the biomass at the end of the exposure period minus the biomass at the start of the exposure period.
- Duration:
- 72 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: AUC or Area Under Curve according to EU Method C.3
- Remarks:
- means the integral of the biomass
- Duration:
- 72 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield (according to OECD Guideline 201)
- Remarks:
- It is defined as the biomass at the end of the exposure period minus the biomass at the start of the exposure period.
- Results with reference substance (positive control):
- NOEC 72 h = 0.04m/L (95 IC = not deteminable)
LOEC 72 h = 0.063m/L (95 IC = not deteminable)
72h ErC50 = 1.8mg/L (95 IC = 1.4 - 2.3mg/L)
72h EbC50 = 0.55m/L (95 IC = 0.48 - 0.62 mg/L)
72h EyC50 = 0.35mg/L (95 IC = 0.30 - 0.4 mg/L)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- All validity criteria were met.
The EC50s of potassium dichromate were determined in a current reference test. For the estimation of the EC50s of the positive control, the fits showed sufficient statistical correspondence of the data with the dose-response-equation, The values were within the normal range of the laboratory.
In the lowest concentrated treatment (1 mg/L), significant inhibition was observed. Therefore no NOEC can be stated.
In the main study, replicate 2 of the control and in the reference study, replicate 3 of the control showed abnormally large absorptions. Upon inspection of the replicates, a contamination was discovered. Therefore, these replicates were excluded from the evaluation.
The result of the test can be considered valid. - Executive summary:
The study was performed using three concentrations containing 1, 10 and 100 mg/L. The treatments were used to incubate the unicellular freshwater green alga Desmodesmus subspicatus for a period of 72 hours. The cell concentration of each replicate was determined by measuring the absorption of the cuvettes at 440 nm every 24 hours with a spectral photometer. With these measured values, the number of cells was calculated (linear correlation between cell concentration and absorption given). Then the growth rate µ, the area under the growth curve (AUC) and the Yield were determined.
The EC50s of potassium dichromate were tested in a current reference test. The values lay within the normal range of the laboratory.
The following results for the test item was determined:
Endpoint NOEC LOEC EC50 Growth Rate <1mg/L 1mg/L approx. 10 mg/L AUC <1mg/L 1mg/L 1 -10 mg/L Yield <1mg/L 1mg/L <1mg/L
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