Reaction product of tetrahydro-2,5-dimethoxy furan, ethanol and water

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

Fresenius

Specific details on test material used for the study:

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: At ambient temperature, protected from light
- Solubility and stability of the test substance in the solvent/vehicle: Amber liquid, indefinitely soluble
at 20°C

Analytical monitoring:

yes

Details on sampling:

The level of TOC measured in freshly prepared test media samples and aged (after 1 day) test media samples, were compared against the carbon content of the test item measured

Vehicle:

no

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Source: Aquahandel.de (Holtenser Weg 6a, 37186 Moringen)

Test type:

semi-static

Limit test:

no

Total exposure duration:

96 h

Test temperature:

21.0 - 22.8 °C

pH:

8.23 - 8.66

Dissolved oxygen:

7.0 - 9.2 mg O2/L

Nominal and measured concentrations:

Recoveries of >69 to >82 % of the initial values measured at time t0 were obtained in the treatments after 24 hr of incubation in one pair of treatments, and 95% to 109% in a second pair of values. Measured concentrations of the treatment 4.55 mg/L deviated more than 20% from the nominal values within the first pair of values. Based on this, the reported effect concentrations should refer to mean measured concentrations (geometric mean) of the measured concentrations.

Details on test conditions:

TEST SYSTEM
- Fill volume: 3 L
- Aeration: yes
- No. of organisms per vessel: 7
- The fish were not fed 24 hr prior to testing and during the whole test

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: municipal drink water
- Intervals of water quality measurement: At the beginning and every day of the study the pH-values, oxygen content and the temperature were measured in both the freshly prepared test solutions and those being in contact with the fish for each period of 24 hr.

OTHER TEST CONDITIONS
- Adjustment of pH: no

EFFECT PARAMETERS MEASURED:
Daily the test solutions were examined for dead animals, and any abnormal behaviour of the test animals was recorded.

TEST CONCENTRATIONS
- Test concentrations: 2.07, 4.55, 10.0, 22.0, 48.4 mg/L (nominal concentrations)

Reference substance (positive control):

no

Remarks:

Within the analytical part, the test item served as a reference item.

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

48.32 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Reported statistics and error estimates:

ToxRatPro Version 3.2.1

Sublethal observations / clinical signs:

Results Main Test:

Nominal Concentration [mg Test Item per Liter of Tap Water]	Mortality [%] after
	1d	2d	3d	4d (96 hr)
Control (0)	0 (0 of 7)	0 (0 of 7)	0 (0 of 7)	0 (0 of 7)
2.07	0 (0 of 7)	0 (0 of 7)	0 (0 of 7)	0 (0of 7)
4.55	0 (0 of 7)	0 (0 of 7)	0 (0 of 7)	0 (0 of 7)
10.0	0 (0 of 7)	28.6 (2 of 7)	28.6 (2 of 7)	42.9 (3 of 7)
22.0	0 (0 of 7)	0 (0 of 7)	0 (0 of 7)	0 (0 of 7)
48.4	0 (0 of 7)	28.6 (2 of 7)	42.9 (3 of 7)	57.1 (4 of 7)

 

 

Calculated LC-Values (after 96 hr):

 

On the Basis of the nominal Concentrations [mg/L]	On the Basis of the measured Concentrations [mg/L]
LC10           7.09   LC20         13.98                                                          LC50         51.13                                                          Mortality  LOEC n.d. (>10) NOEC n.d. (≥ 4.55)	LC10           6.02   LC20         12.30                                                          LC50         48.32                                                          Mortality  LOEC n.d. (>9.04) NOEC n.d. (≥ 3.66)

 

 

 

 Observations on the Behaviour of the Fish (Main Test):

 

Time (h)	Effects / Observations
1	In general animals were nervous; no further symptoms visible
2	In general animals were nervous; no further symptoms visible
4 – 6	In general animals were nervous; no further symptoms visible
18 – 20	In general animals were nervous; no further symptoms visible
24	In general animals were nervous; no further symptoms visible
28 – 30	At 49.1 mg/L one fish disoriented, one immobile
42 – 44	At 10 mg/L and 49.1 mg/L 2 fish dead; no further effects visible
48	At 48.4 mg/L one fish immobile
48-66	At 48.4 mg/L one fish immobile
72	At 48.4 mg/L one fish dead; no further symptoms visible; 2 hrs later at 22 mg(L one fish immobile
96	At 10 mg/L and 48.4 mg/L one fish dead, at 22 mg/L one fish immobile

 

Validity criteria fulfilled:

yes

Conclusions:

In the test on acute toxicity to Danio rerio, the EC50 was determined to be 48.32 mg/L (based on measured concentrations).

Executive summary:

„Reaction Product from Tetrahydro-2,5-dimethoxyfurane, Ethanol and Water“ was tested for acute toxicity towards fish according to OECD Guideline No.203 (1992) using zebra fish. In order to investigate the influence of the test item towards the fish, the animals were exposed to up to 100 mg of the test item per litre of test solution (screening test). Under the conditions used for the test, toxic effects of the test item towards fish were observed at concentrations ≥ 10 mg/L.The stability of the test item in the test was checked using TOC analyses. As a conclusion of the analytical part of this study, recoveries of the TOC concentrations in the treatments with the test item determined after 24hr of incubation were not all at ≥ 80% of those determined in freshly prepared treatments. TOC in the Blank treatments after 24 hr (48hr) of incubation with the fish varied at high percentage, and thus should not be subtracted directly from the values obtained with the test item.

Description of key information

„Reaction Product from Tetrahydro-2,5-dimethoxyfurane, Ethanol and Water“ was tested for acute toxicity towards fish according to OECD Guideline No.203 (1992) using zebra fish. In order to investigate the influence of the test item towards the fish, the animals were exposed to up to 100 mg of the test item per litre of test solution (screening test). Under the conditions used for the test, toxic effects of the test item towards fish were observed at concentrations ≥ 10 mg/L. The stability of the test item in the test was checked using TOC analyses. As a conclusion of the analytical part of this study, recoveries of the TOC concentrations in the treatments with the test item determined after 24 h of incubation were not all at ≥ 80% of those determined in freshly prepared treatments. TOC in the Blank treatments after 24 h (48 h) of incubation with the fish varied at high percentage, and thus should not be subtracted directly from the values obtained with the test item.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

48.32 mg/L