Registration Dossier
Registration Dossier
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EC number: 600-254-8 | CAS number: 10195-54-7
Endpoint summary
Administrative data
Description of key information
The biodegradation of the test substance was 77% after 28 days in the test according to OECD 301D. Therefore, the test substance was readily biodegradable in the closed bottle test.
Additional information
In order to assess the biotic degradation, a ready biodegradability test was performed which allows the biodegradability to be measured in an aerobic aqueous medium. The ready biodegradability was determined in the Closed Bottle test according to OECD 301D and EEC Guidelines, and in compliance with the OECD principles of Good Laboratory Practice. In the biodegradability tests, microorganisms are inoculated into a chemically defined liquid medium containing the test substance as sole carbon and energy source. The test compound is exposed to the inoculum, consisting of relatively low numbers of microorganisms from an activated sludge plant, under aerobic conditions for a period of at least 28 days. The test substance caused no reduction in the endogenous respiration. The test substance is therefore considered to be non-inhibitory to the inoculum. The biodegradation of the test substance was 77% after 28 days. The percentage biodegradation reached 60 % within the 10-day window. Hence, the test substance was readily biodegradable in the Closed Bottle test (28 days) and should therefore be classified as readily biodegradable. The reference substance, sodium benzoate, was degraded 86 % of its theoretical oxygen demand after 28 days.
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