5-Benzofuranacetic acid, 2,3-dihydro-, methyl ester

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

other information

Study period:

from 16-NOV-2001 to 25-FEV-2002

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Test performed according to OECD 203 (with some deviation), but the validity criteria is not fulfilled (20% mortality in the control group)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

no analytical monitoring, only 3 tested groups

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

yes

Remarks:

no analytical monitoring, only 3 tested groups

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

ADHBFA-Me

IUPAC Name:

ADHBFA-Me

Test material form:

other: liquid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: the stock solution was prepared using 450.8 mg of test item mad up to 4500 mL of test medium, stirred during 4 hours

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Danio rerio
- Source: no data
- Length at study initiation (length definition, mean, range and SD): 3.28 cm (min: 2.9 cm, max: 3.5 cm)
- Feeding during test: no


ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions (same as test or not): no data
- Type and amount of food: no data
- Feeding frequency: no data
- Health during acclimation (any mortality observed): 0% mortality

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

none

Test conditions

Hardness:

90 mg of CaCO3 per litre of test medium

Test temperature:

21.0 °C to 23.1 °C

pH:

8.06 to 8.29

Dissolved oxygen:

98 to 105 mg/L

Nominal and measured concentrations:

0, 1, 10 and 100 mg/L (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel: tank of at least 4-litre capacity
- Type: supplied with cover
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): one
- No. of vessels per control (replicates): one
- Biomass loading rate: maximum 1 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water
- Culture medium different from test medium: no data
- Intervals of water quality measurement: regularly (no other information)

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12hr/12hr
- Light intensity: no data

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- mortality (at 2-4 hrs and then at 24, 48, 72 and 96 hrs)
- clinical observations (at 2-4 hrs and then at 24, 48, 72 and 96 hrs)

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10
- Justification for using less concentrations than requested by guideline: restricted test

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

10 - 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: at 100 mg/L equilibrium problem, pigmentation problems and breating problems at 0-4 hrs. Nothing to report thereafter.
- Mortality of control: 1 death at 24 hrs and 2 death at 48 hours (20% mortality)
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none
- Effect concentrations exceeding solubility of substance in test medium: no data

Applicant's summary and conclusion

Validity criteria fulfilled:

no

Remarks:

20% mortality in control

Conclusions:

Danio rerio 96-hr LC50 is between 10 and 100 mg/L