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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
Experimental phase 13 April to 15 April 2010. Report issued: 06 May 2010.
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Screening study using a method similar to OECD 202, but without any analysis and with only a summary report.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010
Report date:
2010

Materials and methods

Test guideline
Guideline:
other:
Version / remarks:
The study was a screening study based on OECD 202.
Deviations:
yes
Remarks:
No analysis of test solutions undertaken.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
4-methyl-2,6-bis(phenylamino)pyridine-3-carbonitrile
EC Number:
810-804-0
Cas Number:
51566-48-4
Molecular formula:
C19H16N4
IUPAC Name:
4-methyl-2,6-bis(phenylamino)pyridine-3-carbonitrile
Test material form:
solid: particulate/powder

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Remarks:
Acetone

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 3.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

The effect of the test substance on Daphnia magna is presented in the table below:

 Nominal Concentration of Test Material (mg/l)  % Daphnia Immobilised at 48 Hours
 Solvent Control  10
 0.01  0
 0.032  0
 0.1  0
 0.32  0
 1.0  0
 3.2  0
 EC50 (mg/l)  > 3.2
 No Observed Effect Concentration (mg/l)  3.2

Applicant's summary and conclusion

Validity criteria fulfilled:
not applicable
Conclusions:
The 48 hour EC50 to Daphnia magna based on nominal concentrations of the test substance was > 3.2 mg/l.
Executive summary:

Introduction

The acute toxicity of the test susbtance to Daphnia magna was assessed in a screening test base on OECD Method 202: Daphnia sp. Acute Immobilisation Test

Method

Five Daphnia magna per dose were exposured to test material at concentrations between 0.01 and 3.2 mg/l. A solvent control was also included which contained 10 Daphnia magna. The Daphnia were exposed for 48 hours and at the end of the exposure period assessed for mobility.

Results and Conclusion

The 48 hour EC50 to Daphnia magna based on nominal concentrations of the test substance was > 3.2 mg/l.