Pentyl phenylacetate

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Experiment test result performed using standard test guideline

Justification for type of information:

Data is from ABITEC lab report.

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Principles of method if other than guideline:

Short term toxicity of pentyl 2-phenylacetate (5137-52-0) to aquatic algae was performed according to the OECD guideline in a static system.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (IUPAC name): pentyl 2-phenylacetate
- Common name: Amyl phenylacetate
- Molecular formula: C13H18O2
- Molecular weight: 206.283 g/mol
- Smiles notation: C(=O)(Cc1ccccc1)OCCCCC
- InChl: 1S/C13H18O2/c1-2-3-7-10-15-13(14)11-12-8-5-4-6-9-12/h4-6,8-9H,2-3,7,10-11H2,1H3
- Substance type: Organic

Analytical monitoring:

not specified

Vehicle:

yes

Details on test solutions:

The stock solution (10.0 mg/L) was prepared by dissolving colourless liquid in OECD growth medium. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Common name:
- Strain: 86.81 SAG
- Source (laboratory, culture collection): Institute of botany of the ASCR
- Initial biomass concentration: 5x10(3) cells /ml
- Method of cultivation: No data available

ACCLIMATION - No data available
- Acclimation period:
- Culturing media and conditions (same as test or not):
- Any deformed or abnormal cells observed:

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Remarks on exposure duration:

±1 hour

Test temperature:

23±2°C

pH:

Test at highest concentration: 7.9 (no change during tests)
Control: 8.0 (changed to 8.8 during test)

Nominal and measured concentrations:

0, 2,4,8,16 and 32 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 50/ 100 ml Glass vessel
- Type (delete if not applicable): closed (with air permeable stopper)
- Sample volume: 15.0/ 50 ml
- Initial cells density: 5x10(3) cells/ml
- No. of vessels per concentration (replicates): 3

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: Continuous
- Light intensity and quality: 6000-8000 lx

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: microscope with counting chamber Cyrus I or electronic particle counter.
- Other: ErC50 was calculated using non-linear regression by the software Prism 4.0

Reference substance (positive control):

yes

Remarks:

Potassium dichromate (K2Cr2O7)

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

17.6 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 95% CL: 16.3-19.0

Results with reference substance (positive control):

Results with reference substance valid
- EC50: 0.75 mg/L

Validity criteria fulfilled:

not specified

Conclusions:

The median effective concentration (EC50) for the test substance, pentyl 2-phenylacetate (5137-52-0), in Desmodesmus subspicatus was determined to be 17.6 mg/L on the basis of effects on growth rate in a 72 hour study.

Executive summary:

Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance pentyl 2-phenylacetate (5137-52-0) according to OECD Guideline 201.

The test substance was dissolved in OECD growth medium and tested at the concentrations 0, 2,4,8,16 and 32 mg/L. Effects on the growth rate of the organism were studied.

The median effective concentration (EC50) for the test substance, pentyl 2-phenylacetate (5137-52-0), in Desmodesmus subspicatus was determined to be 17.6 mg/L. This EC50 value indicates that the substance is likely to be hazardous to aquatic algae and can be classified as aquatic chronic 3 category as per the CLP criteria. But as the chemical was readily biodegradable in water thus it can be concluded that the chemical was nontoxic and not classified as toxic asper the CLP classification criteria. 

Description of key information

Toxicity to aquatic algae and cyanobacteria :

Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substancepentyl 2-phenylacetate (5137-52-0)according to OECD Guideline 201.

The test substance was dissolved in OECD growth medium and tested at the concentrations0, 2,4,8,16 and 32 mg/L. Effects on the growth rate of the organism were studied.

The median effective concentration (EC50) for the test substance,pentyl 2-phenylacetate (5137-52-0), in Desmodesmus subspicatus was determined to be 17.6 mg/L.This EC50 value indicates that the substance is likely to be hazardous to aquatic algae and can be classified as aquatic chronic 3 category as per the CLP criteria. But as the chemical was readily biodegradable in water thus it can be concluded that the chemical was nontoxic and not classified as toxic asper the CLP classification criteria. 

Key value for chemical safety assessment

EC50 for freshwater algae:

17.6 mg/L

Additional information

Toxicity to aquatic algae and cyanobacteria :

Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substancepentyl 2-phenylacetate (5137-52-0)according to OECD Guideline 201.

The test substance was dissolved in OECD growth medium and tested at the concentrations0, 2,4,8,16 and 32 mg/L. Effects on the growth rate of the organism were studied.

The median effective concentration (EC50) for the test substance,pentyl 2-phenylacetate (5137-52-0), in Desmodesmus subspicatus was determined to be 17.6 mg/L.This EC50 value indicates that the substance is likely to be hazardous to aquatic algae and can be classified as aquatic chronic 3 category as per the CLP criteria. But as the chemical was readily biodegradable in water thus it can be concluded that the chemical was nontoxic and not classified as toxic asper the CLP classification criteria.