Arctium lappa, ext.

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

disregarded due to major methodological deficiencies

Study period:

2008

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Unsuitable test system. The dissolved oxygen concentration was not > 3 mg/L at the end of the test as recommended by the guideline.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

part 1

Deviations:

yes

Remarks:

The oxygen concentration was not >= 60% of the saturation value

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Arctium lappa, ext.

EC Number:

281-658-8

EC Name:

Arctium lappa, ext.

Cas Number:

84012-13-5

IUPAC Name:

Arctium lappa, ext.

Details on test material:

- Name of test material (as cited in study report): Klettenfruchtextrakt conc.
- Physical state / colour / odour: viscous liquid, golden brown, fruity odour

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Strain: Clone 5
- Source: Umweltbundesamt Berlin, Versuchsfeld Mariendorf
- Food type: Desmodesmus subspicatus, pure culture
- Frequency: daily

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

10.3 °dH, 184 mg CaCO3/L

Test temperature:

21.4 - 22.0°C

pH:

6.9 - 7.9

Dissolved oxygen:

0.9 - 8.2 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: beakers
- Fill volume: 40 mL
- Renewal rate of test solution: daily
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: drinking water, dechlorinated
- Alkalinity: 0.12 mmol/L
- Na/K ratio: ca. 4:1
- Ca/Mg ratio: 3.8:1

OTHER TEST CONDITIONS
- Photoperiod: 16 h light, 8 h dark
- Light intensity: 20 µE/m2 s

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

117 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

164 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 95% CI: 135-197 mg/L

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

263 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

- Abnormal responses: none

Reported statistics and error estimates:

Probit analysis

Any other information on results incl. tables

Mortality [%] after 24/48 h:

Concentration [mg/L]	592	394	263	175	117	78	Control [24 h]
Mortality [%]	100/100	90/100	80/100	35/75	0/0	5/5	0/0

 

Applicant's summary and conclusion

Validity criteria fulfilled:

no