2-methoxy-5-nitroanilinium chloride

Endpoint summary

Description of key information

Short-term toxicity to aquatic invertebrates

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity to aquatic invertebrate was predicted 2-methoxy-5-nitroanilinium chloride (CAS: 67827-72-9). EC50 mobility value was estimated to be 101.7508 mg/l for Daphnia magna for 48 hrs duration. It was concluded that 2-methoxy-5-nitroanilinium chloride (CAS: 67827-72-9) was likely to be not toxic to aquatic invertebrate.

Toxicity to aquatic algae and cyanobacteria

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity to aquatic algae was predicted 2-methoxy-5-nitroanilinium chloride (CAS: 67827-72-9). EC50 growth rate value was estimated to be 135.836 mg/l for Pseudokirchneriella subcapitata for 72 hrs duration. It was concluded that 2-methoxy-5-nitroanilinium chloride (CAS: 67827-72-9) was likely to be not toxic to aquatic algae.

Additional information

Short-term toxicity to aquatic invertebrates

Following studies of target chemical and structurally similar read across includes predicted data and experimental data to conclude the toxicity extent of 2-methoxy-5-nitroanilinium chloride (CAS: 67827-72-9) towards invertebrate is summarized as follows:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity to aquatic invertebrate was predicted 2-methoxy-5-nitroanilinium chloride (CAS: 67827-72-9). EC50 mobility value was estimated to be 101.7508 mg/l for Daphnia magna for 48 hrs duration. It was concluded that 2-methoxy-5-nitroanilinium chloride (CAS: 67827-72-9) was likely to be not toxic to aquatic invertebrate.

The above predicted data of target chemical is supported by the experimental study of structurally similar read across 3 – Nitrobenzene sulfonic acid (CAS No. 127-68-4) from the experimental ABITEC lab report 2016, suggests that theDetermination of the inhibition of the mobility of daphnids was carried out with the substance 3 – Nitrobenzene sulfonic acid according to OECD Guideline 202. The test substance was tested at the limit concentration 100 mg/l. Effects on immobilisation were observed for 48 hours. Based on the % inhibition of daphnia magna, due to the exposure of chemical 3 – Nitrobenzene sulfonic acid, the IC8 was 100 mg/l.

This value indicates that the substance is likely to be non-hazardous to aquatic invertebrates and cannot be classified as toxic as per the CLP criteria.

Another supporting experimental study for the structurally similar read across Sulfanilic acid (121-57-3) from the publication Water Pollution Control Federation 1965, also suggests that the Short term toxicity of test chemical sodium 4-chloro-3-nitrobenzene-1-sulfonate was determined in Daphnia magna which were cultured in laboratory from starting culture originally obtained from Put in Bay, Ohio. The experiment was static test and medium used was glasswool filtered university lake water. Median tolerance limit(TLm) of test chemical sodium 4-chloro-3-nitrobenzene-1-sulfonate was observed in daphnia magna at different time intervals. At 24 hrs and 48 hrs it was 8665 mg/L and at 72 hrs TLm was 6017 mg/L on the basis of immobilization. On the basis of TLm value at different time interval it is concluded that this test chemical is considered as "not classified" according to CLP criteria.

Thus based on the effect concentrations which is in the range 100 mg/l to 8665 mg/l give the conclusion that test substance 2-methoxy-5-nitroanilinium chloride (CAS: 67827-72-9) was likely to be not toxic to aquatic invertebrate at environmentally relevant concentrations and can be considered to be “not classified” as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria

Following studies of target chemical and structurally similar read across includes predicted data and experimental data to conclude the toxicity extent of 2-methoxy-5-nitroanilinium chloride (CAS: 67827-72-9) towards aquatic algae is summarized as follows:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity to aquatic algae was predicted 2-methoxy-5-nitroanilinium chloride (CAS: 67827-72-9). EC50 growth rate value was estimated to be 135.836 mg/l for Pseudokirchneriella subcapitata for 72 hrs duration. It was concluded that 2-methoxy-5-nitroanilinium chloride (CAS: 67827-72-9) was likely to be not toxic to aquatic algae.

The above predicted data of target chemical is supported by the experimental study of structurally similar read across 3 – Nitrobenzene sulfonic acid (CAS No. 127-68-4) from the experimental ABITEC lab report 2016, suggests that theFreshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance 3 – Nitrobenzene sulfonic acid according to OECD Guideline 201.

The test substance was dissolved in OECD growth medium and tested at the limit concentration 100 mg/L. Effects on the growth rate of the organism were studied.

The NOEC for the test substance, 3 – Nitrobenzene sulfonic acid, sodium salt, in Desmodesmus subspicatus was determined to be 100 mg/L on the basis of effects on growth rate in a 72 hour study.

This value indicates that the substance is likely to be non-hazardous to aquatic algae and cannot be classified as toxic as per the CLP criteria.

Thus based on the effect concentrations which is in the range 100 mg/l to 135.836 mg/l give the conclusion that test substance 2-methoxy-5-nitroanilinium chloride (CAS: 67827-72-9) was likely to be not toxic to aquatic algae at environmentally relevant concentrations and can be considered to be “not classified” as per the CLP classification criteria.