Pyrazino[2,1-a]isochinolin-4-on, 4H-, 1,2,3,6,7,11b-hexahydr o-, Diphosphat

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Oct 16, 2002 - Jan 24, 2003

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP study performed according to OECD TG 301D.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Automatically generated during migration to IUCLID 6, no data available

IUPAC Name:

Automatically generated during migration to IUCLID 6, no data available

Test material form:

other: solid

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure):
activated sludge, micro organisms from a domestic waste water treatment plant

- Origin
Effluent of a municipal sewage treatment plant, Südhessische Gas und Wasser AG, Darmstadt

-Treatment:
Filtration through a coarse folded filter

- Concentration:
5 mL/L

- Preparation:
Aearation for 5 days

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

According to guideline

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

Preliminary study:

The biodegradation in % was calculated based on the ThOD of 1085 mg 02/mg test item.

Test performance:

The reference item sodium benzoate was sufficiently degraded to 87%, 89% and 82% after an incubation of 14, 21 and 28 days respectively.
The validity criterion that the degradation should be > 60 % after 14 d was fulfilled.

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

5

Sampling time:

28 d

Details on results:

Results of the Toxicity Control
In the toxicity control containing both the test item and reference 48% biodegradation was noted within 14 days and 41% biodegradation was determined after 28 days of incubation.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: not readily biodegradable

Conclusions:

The degradation rate of the test material did not reach 60 % within the 10-day window and after 28 days of incubation. Thus the test item is not readily biodegradable.

Executive summary:

Purpose

The test item was investigated for its ready biodegradability in a Closed Bottel Test over a period of 28 days. The biodegradation was followed by the decrease in the concentration of dissolved oxygen. As a reference item sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control.The GLP study was performed according to OECD TG 301D.

Result

Biodegradation of test item

After correction of the mean biochemical oxygen demand of the inoculum controls at the end of the 28 -day exposure period a degradation of 5 % as found.

Biodegradation of sodium benzoate

The reference item was sufficiently degraded to 87 % after 14 days (> 60%), thus confirming the suitability of the used activated sludge inoculum.

Biodegradation in the Toxicity Control

In the toxicity control containing both, the test item and the reference item, 48 % biodegradation was noted within 14 days and 41 % biodegradation was determined after 28 days of incubation. Thus, the test item can be assumed to be not inhibitory on the activated sludge micro organisms.

Conclusion

The degradation rate of the test material did not reach 60 % within the 10 -day window and after 28 days of incubation. Thus the test item is not readily biodegradable.