Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 223-296-5 | CAS number: 3811-73-2
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.12 (Mutagenicity - In Vivo Mammalian Erythrocyte Micronucleus Test)
- GLP compliance:
- yes
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- Pyridine-2-thiol 1-oxide, sodium salt
- EC Number:
- 223-296-5
- EC Name:
- Pyridine-2-thiol 1-oxide, sodium salt
- Cas Number:
- 3811-73-2
- Molecular formula:
- C5H5NOS.Na
- IUPAC Name:
- sodium (1-oxo-1λ⁵-pyridin-2-yl)sulfanide
- Details on test material:
- - Analytical purity: 92.5%
- Lot/batch No.: SB-D010010
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- NMRI
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River WIGA GmbH
- Age at study initiation: 9 weeks
- Weight at study initiation: male 28.3-29.5g female 26.3-27.9g
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Frequency of treatment:
- 1
- Post exposure period:
- -Post exposure period: 24 and 48h
Doses / concentrations
- Remarks:
- Doses / Concentrations:
400, 482, 580 mg/kg bw
Basis:
- No. of animals per sex per dose:
- 5m + 5f per dose and sampling time
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- Cyclophophamide (monohydrate), 40 mg/kg bw.
Examinations
- Tissues and cell types examined:
- -Bone marrow
- Evaluation criteria:
- Polychromatic/normochromatic erythrocytes ratio
Micronucleated erythrocytes
Results and discussion
- Additional information on results:
- No statistically significant increase in the amounts of micronucleated polychromatic erythrocytes was observed at any dose tested compared to the negative controls, neither 24 nor 48 hours after treatment, neither for males nor for females. Bioavailability of the test substance was proven by mortality and by cytotoxicity at the high dose.
Applicant's summary and conclusion
- Conclusions:
- The test substance does not produce relevant increases of the numbers of micronuclei in polychromatic erythrocytes after in vivo treatment of mice of either sex of the test strain at doses of 400, 482 and 580 mg/kg bw. The information contained within this robust summary document comes from studies which are in the ownership of Arch Chemicals Inc. and which are protected in several regions globally. This information may not be used for any purpose other than in support of the Chemical safety Report submitted by Arch Chemicals Inc. under Regulation EC 1907/2006.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
