Reaction mass of dodecyl acrylate and tridecyl acrylate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1988-07-25 to 1988-07-29

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Meets National Standard Methods (DIN) with acceptable restrictions Read across was performed with Laurylacrylat 12/14 (mixture of laurylacrylate (CAS No.: 2156-97-0) and tetradecylacrylate (CAS No.: 21643-42-5) . Please refer to IUCLID section 13 for read across justification.

Qualifier:

according to guideline

Guideline:

other: German industrial standard test guideline DIN 38 412, part L 15, JUNE 1982

Deviations:

no

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The dose was chosen based on a range finding study.
- The product was added to the test water without any pretreatment; subsequently the fish were placed into the aquaria. Based on the results of a range finding study (LC 50 after 96 h: between 100 and 1000 mg/L) the concentrations, spaced by a factor of about 2.2, were fixed as follows: 100, 215, 464 and 1000 mg/L.
- Vehicle/solvent: none
- Stability of the test chemical solutions: no data

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Goldorfe
- Strain: Leuciscus Idus L., Goden variety
- Source: Fischzucht Paul Eggers, 2354 Hohenwestedt, Germany
- Length at study initiation: 7.7 cm (Range: 6.9 -8.9)
- Weight at study initiation: 4.3 g (Range: 2.8 - 6.6)
- Feeding during test : 1 day before and during exposure (withdrawal of food)

ACCLIMATION
- Acclimation period: 3 days
- Acclimation conditions: same as test
- Type and amount of food: yes, ad libitium (Growing Feed F/B 50, SSniff Spezialdiaeten GmbH, D-4770 Soest, FRG)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

2.5 mmol/L

Test temperature:

20 °C

pH:

7.5 - 7.9

Dissolved oxygen:

7.3 - 8.5 mg/L

Nominal and measured concentrations:

Nominal concentration: 0, 100, 215, 464, 1000 mg/L
No concentrations were measured.

Details on test conditions:

TEST SYSTEM
- Test vessel: all-glass aquaria
- Type: open
- Material, size, headspace, fill volume: 30x 22 x 24 cm, 10 L
- Aeration: aeration with oil-free air
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 4.3 g fish/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, part 11
- Ca/mg ratio: 4:1
- Intervals of water quality measurement: 1, 4, 24, 48, 72, 96 hours

OTHER TEST CONDITIONS
- Photoperiod: 16:8 hour light/dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, symptoms

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

215 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

ca. 460 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

215 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Biological Observations: At the test concentration of 464 mg/L a number of fish showed apathy after 48, 72 and 96 hours. At the highest substance concentration (1000 mg/L) tumbling and narcotic-like state were observed after 24 h among the fish.
- Lowest test substance concentration causing 100% mortality: 1000 mg/L
- Mortality of controls: none

Results with reference substance (positive control):

Positive control of animals conducted with chloroacetamide: LC 50 after 48 h: about 26 mg/L
This lethal concentration corresponds to the normal sensitivity.

Reported statistics and error estimates:

Probit analysis according to Finney DJ (1971). Probit Analysis, Cambr. Univ. Press, 3rd Ed.

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

other information

Reason / purpose for cross-reference:

read-across source

Description of key information

No acute fish toxicity study is available on the registered substance (Reaction mass of C12-C13 acrylates, EC Number 911-296-4). However, experimental data are available on an analogue substance (Reaction mass of C12-C14 acrylates, CAS 84238-60-8).

In an experimental study conducted according to the DIN 38 412, Part L 15 (June 1982), the LC50 was determined to be ca. 460 mg/L and the NOEC was equal to 215 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

460 mg/L