Fatty acids, C16-18 and C18-unsatd., reaction products with glycidyl neodecanoate and 7-oxabicyclo[4.1.0]hept-3-ylmethyl 7-oxabicyclo[4.1.0]heptane-3-carboxylate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

17 February 2016 to 22 March 2016

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

The test substance is listed as ZEF 6099/100. The test substance, a brown liquid, is the reaction product of C16-C18 and C18 unsaturated fatty acids, with (3,4-epoxycyclohexyl) methyl 3,4-epoxycyclohexylcarboxylate and neodecanoic fatty acid, oxiranylmethylester. The test substance was obtained from the sponsor and was stored at room temperature (16.1 – 20.9°C) in a closed vessel dark and dry.

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

Activated sludge from a biologic sewage treatment plant, treating mostly domestic sewage. The sludge was taken from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant, Im Altenschemel, NW-Lachen-Speyerdorf on 19 Feb. 2016.

Duration of test (contact time):

28 d

Initial conc.:

20 mg/L

Based on:

TOC

Initial conc.:

27.6 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

CO2 evolution

Reference substance:

aniline

Key result

Parameter:

% degradation (CO2 evolution)

Value:

48

Sampling time:

28 d

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

The test substance is considered as “not readily biodegradable”. The test substance reached 48% biodegradation after 28 d, falling short of the target 60% degradation within 28 d. At the end of the 28 d test, no degradation plateau was reached yet, indicating further biodegradation is possible. Because the test substance is a mixture, the 10-day-window biodegradation target does not apply.

Description of key information

The key study was a CO₂ evolution test conducted in accordance with OECD Guideline 301B. Based upon test results (48% degradation in 28 days), the substance was reported as “not readily biodegradable”, below the passing criterion of 60% for ready biodegradability. The degradation rate of 48% in 28 days is evidence of inherent, primary biodegradability (> 20% biodegradation) and significant mineralisation (> 40% degradation). Therefore, the test substance was also reported as "very likely inherently biodegradable".

Key value for chemical safety assessment

Biodegradation in water:

inherently biodegradable