Morpholine, 4-[(diethoxymethylsilyl)methyl]-

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2007-06-13 to 2007-09-25

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: OECD Guideline under GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Version / remarks:

(92/69/EWG, C.4-D)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

480-370-1

EC Name:

-

Cas Number:

21743-27-1

Molecular formula:

Hill formula: C11H25NO4Si CAS formula: C11H25NO4Si

IUPAC Name:

4-((Triethoxysilyl)methyl)morpholine

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): sewage plant Darmstadt, Germany´
- Preparation of inoculum for exposure: washed (3 times);
- Concentration of sludge: 1.5 g dry weight per litre (determinded by ratio of wet to dry weight of sludge)
- Pretreatment: Preincubation for 2 days in test water

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

100 mg/L

Based on:

formulation

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: Reconstituted test water
- Additional substrate: no
- Solubilising agent (type and concentration if used): no
- Test temperature: 22 °C
- pH: 7.6-7.9
- pH adjusted: no
- Aeration of dilution water: not given in the report
- Continuous darkness: yes


TEST SYSTEM
- Culturing apparatus: 500 mL flasks
- Number of culture flasks/concentration: 2
- Details of trap for CO2 and volatile organics if used: carbon dioxide was absorbed in an aqueous solution of potassium hydroxide


SAMPLING
- Sampling frequency: daily
- Sampling method: BOD


CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes (HgCl2 poisoned)
- Toxicity control: yes
- Reference substance: yes, Sodium benzoate

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (O2 consumption)

Value:

54

Sampling time:

28 d

Remarks on result:

other: with consideration of nitrification; 10 day window failed

% Degradation 2

Parameter:

% degradation (O2 consumption)

Value:

61

Sampling time:

28 d

Remarks on result:

other: witout consideration of nitrification; 10 day window failed

Details on results:

With consideration of nitrification :
14 d: 36%
28 d: 54%

Toxicity control (with consideration of nitrification):
14 d: 64%
28 d: 70%

Results with reference substance:
14 d: 89%
28 d: 95%

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: not readily biodegradable

Conclusions:

The test item (N-Morpholinomethyl)triethoxysilane is considered not to be readily biodegradable. From results of the toxicity control it can be assumed that the test item is not inhibitory on the activated sludge micro organisms. The positive control with sodium benzoate worked as expected.

Executive summary:

(N-Morpholinomethyl)triethoxysilane was investigated for its ready biodegradability in a manometric respiratiory test over a period of 28 days (OECD Guideline 301 F). The biodegradation was followed by the oxygen uptake of the micro-organisms during exposure. The test item

(N-Morpholinomethyl)triethoxysilane

is considered not to be readily biodegradable. From results of the toxicity control it can be assumed that the test item is not inhibitory on the activated sludge micro organisms. The positive control with sodium benzoate worked as expected.