3-methyl-1-phenylpyrazol-5-ylamine

Endpoint summary

Description of key information

Biodegradation in water:

28-days Closed Bottle test following the OECD guideline 301 D to determine the ready biodegradability of the test item. The study was performed at a temperature of 20°C. The test system included control, test item and reference item. Polyseed were used for this study. 1 polyseed capsule were added in 500 ml D.I water and then stirred for 1 hour for proper mixing and functioning of inoculum. This gave the bacterial count as 10E7 to 10E8 CFU/ml. At the regular interval microbial plating was also performed on agar to confirm the vitality and CFU count of microorganism. The concentration of test and reference item (Sodium Benzoate) chosen for both the study was 4 mg/L, while that of inoculum was 32 ml/l. OECD mineral medium was used for the study. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % degradation was calculated using the values of BOD and ThOD for test item and reference item. The % degradation of procedure control (reference item) was also calculated using BOD & ThOD and was determine to be 79.51%. Degradation of Sodium Benzoate exceeds 64.45% on 7 days & 75.30% on 14th day. The activity of the inoculum was thus verified and the test can be considered as valid. The BOD28 value of 3-methyl-1-phenylpyrazol-5-ylamine was observed to be 1.12 mgO2/mg. ThOD was determined by calculation as 1.93 mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to Closed Bottle test was determined to be 58.03 %. Based on the results, the test item, under the test conditions, was considered to be inherently biodegradable in nature.

Biodegradation in water and sediment :

Estimation Programs Interface (EPI Suite, 2017) prediction model was run to predict the half-life in water and sediment for the test compound 3 -methyl-1 -phenyl-1H-pyrazol-5 -amine (CAS No. 1131 -18 -6). If released in to the environment, 12.4% of the chemical will partition into water according to the Mackay fugacity model level III and the half-life period of 3-methyl-1-phenyl-1H-pyrazol-5-amine in water is estimated to be 37.5 days (900 hrs). The half-life (37.5 days estimated by EPI suite) indicates that the chemical is not persistent in water and the exposure risk to aquatic animals is moderate to low whereas the half-life period of 3-methyl-1-phenyl-1H-pyrazol-5-amine in sediment is estimated to be 337.5 days (8100 hrs). However, as the percentage release of test chemical into the sediment is less than 1% (i.e, reported as 0.384%), indicates that 3-methyl-1-phenyl-1H-pyrazol-5-amine is not persistent in sediment.

 

Biodegradation in soil:

The half-life period of 3-methyl-1-phenyl-1H-pyrazol-5-amine (CAS No. 1131 -18 -6) in soil was estimated using Level III Fugacity Model by EPI Suite version 4.1 estimation database (EPI suite, 2017). If released into the environment, 87.2% of the chemical will partition into soil according to the Mackay fugacity model level III. The half-life period of 3-methyl-1-phenyl-1H-pyrazol-5-amine in soil is estimated to be 75 days (1800 hrs). Based on this half-life value of 3-methyl-1- phenyl-1H-pyrazol-5-amine, it is concluded that the chemical is not persistent in the soil environment and the exposure risk to soil dwelling animals is moderate to low.

 

Bioaccumulation: aquatic/sediment

In accordance with the column 2 of Annex IX of the REACH regulation, the bioaccumulation in aquatic species study is scientifically not necessary and does not need to be conducted because the substance has a low potential for bioaccumulation based on log Kow ≤ 3.

Adsorption/ desorption:

The adsorption coefficient Koc in soil and in sewage sludge of test chemical was determined by the Reverse Phase High Performance Liquid Chromatographic method according to OECD Guideline No. 121 for testing of Chemicals. The solutions of the test substance and reference substances were prepared in appropriate solvents. A test item solution was prepared by accurately weighing 4 mg of test item and diluted with ACN up to 10 ml. Thus, the test solution concentration was 400 mg/l. The pH of test substance was 5.0. Each of the reference substance and test substance were analysed by HPLC at 210 nm. After equilibration of the HPLC system, Urea was injected first, the reference substances were injected in duplicate, followed by the test chemical solution in duplicate. Reference substances were injected again after test sample, no change in retention time of reference substances was observed. Retention time tR were measured, averaged and the decimal logarithms of the capacity factors k were calculated. The graph was plotted between log Koc versus log k(Annex - 2).The linear regression parameter of the relationship log Koc vs log k were also calculated from the data obtained with calibration samples and therewith, log Koc of the test substance was determined from its measured capacity factor. The refrence substance Acetanilide, 4-chloroaniline, 4-methylaniline (p-Tolouidine), N-methylaniline, ptoluamide, Aniline, 2,5-Dichloroaniline, 4-nitrophenol, 2 - nitrophenol, 2 nitrobenzamide, 3-nitrobenzamide, Nitrobenzene, 4-Nitrobenzamide, 1-naphthylamine, 1-naphtol, Direct Red 81, Benzoic acid methylester, Carbendazim, Xylene, Ethylbenzene, Toluene, Naphthalene, 1,2,3-trichlorobenzene, Pentachlorophenol, Phenol, N,N-dimethylbenzamide, 3,5-dinitrobenzamide, N-methylbenzamide, Benzamide, phenanthrene, DDT, were chosen having Koc value range from 1.25 to 5.63. The Log Koc value was determined to be 1.835 ± 0.002at 25°C. Thus based on the result it is concluded that the test substance 3-methyl-1-phenylpyrazol-5-ylamine has a low sorption to soil and sediment and therefore has moderate migration potential to ground water.

Additional information

Biodegradation in water:

28-days Closed Bottle test following the OECD guideline 301 D to determine the ready biodegradability of the test item. The study was performed at a temperature of 20°C. The test system included control, test item and reference item. Polyseed were used for this study. 1 polyseed capsule were added in 500 ml D.I water and then stirred for 1 hour for proper mixing and functioning of inoculum. This gave the bacterial count as 10E7 to 10E8 CFU/ml. At the regular interval microbial plating was also performed on agar to confirm the vitality and CFU count of microorganism. The concentration of test and reference item (Sodium Benzoate) chosen for both the study was 4 mg/L, while that of inoculum was 32 ml/l. OECD mineral medium was used for the study. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % degradation was calculated using the values of BOD and ThOD for test item and reference item. The % degradation of procedure control (reference item) was also calculated using BOD & ThOD and was determine to be 79.51%. Degradation of Sodium Benzoate exceeds 64.45% on 7 days & 75.30% on 14th day. The activity of the inoculum was thus verified and the test can be considered as valid. The BOD28 value of 3-methyl-1-phenylpyrazol-5-ylamine was observed to be 1.12 mgO2/mg. ThOD was determined by calculation as 1.93 mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to Closed Bottle test was determined to be 58.03 %. Based on the results, the test item, under the test conditions, was considered to be inherently biodegradable in nature.

Biodegradation in water and sediment :

Estimation Programs Interface (EPI Suite, 2017) prediction model was run to predict the half-life in water and sediment for the test compound 3 -methyl-1 -phenyl-1H-pyrazol-5 -amine (CAS No. 1131 -18 -6). If released in to the environment, 12.4% of the chemical will partition into water according to the Mackay fugacity model level III and the half-life period of 3-methyl-1-phenyl-1H-pyrazol-5-amine in water is estimated to be 37.5 days (900 hrs). The half-life (37.5 days estimated by EPI suite) indicates that the chemical is not persistent in water and the exposure risk to aquatic animals is moderate to low whereas the half-life period of 3-methyl-1-phenyl-1H-pyrazol-5-amine in sediment is estimated to be 337.5 days (8100 hrs). However, as the percentage release of test chemical into the sediment is less than 1% (i.e, reported as 0.384%), indicates that 3-methyl-1-phenyl-1H-pyrazol-5-amine is not persistent in sediment.

 

Biodegradation in soil:

The half-life period of 3-methyl-1-phenyl-1H-pyrazol-5-amine (CAS No. 1131 -18 -6) in soil was estimated using Level III Fugacity Model by EPI Suite version 4.1 estimation database (EPI suite, 2017). If released into the environment, 87.2% of the chemical will partition into soil according to the Mackay fugacity model level III. The half-life period of 3-methyl-1-phenyl-1H-pyrazol-5-amine in soil is estimated to be 75 days (1800 hrs). Based on this half-life value of 3-methyl-1- phenyl-1H-pyrazol-5-amine, it is concluded that the chemical is not persistent in the soil environment and the exposure risk to soil dwelling animals is moderate to low.

 

Bioaccumulation: aquatic/sediment

In accordance with the column 2 of Annex IX of the REACH regulation, the bioaccumulation in aquatic species study is scientifically not necessary and does not need to be conducted because the substance has a low potential for bioaccumulation based on log Kow ≤ 3.

Adsorption/ desorption:

The adsorption coefficient Koc in soil and in sewage sludge of test chemical was determined by the Reverse Phase High Performance Liquid Chromatographic method according to OECD Guideline No. 121 for testing of Chemicals. The solutions of the test substance and reference substances were prepared in appropriate solvents. A test item solution was prepared by accurately weighing 4 mg of test item and diluted with ACN up to 10 ml. Thus, the test solution concentration was 400 mg/l. The pH of test substance was 5.0. Each of the reference substance and test substance were analysed by HPLC at 210 nm. After equilibration of the HPLC system, Urea was injected first, the reference substances were injected in duplicate, followed by the test chemical solution in duplicate. Reference substances were injected again after test sample, no change in retention time of reference substances was observed. Retention time tR were measured, averaged and the decimal logarithms of the capacity factors k were calculated. The graph was plotted between log Koc versus log k(Annex - 2).The linear regression parameter of the relationship log Koc vs log k were also calculated from the data obtained with calibration samples and therewith, log Koc of the test substance was determined from its measured capacity factor. The refrence substance Acetanilide, 4-chloroaniline, 4-methylaniline (p-Tolouidine), N-methylaniline, ptoluamide, Aniline, 2,5-Dichloroaniline, 4-nitrophenol, 2 - nitrophenol, 2 nitrobenzamide, 3-nitrobenzamide, Nitrobenzene, 4-Nitrobenzamide, 1-naphthylamine, 1-naphtol, Direct Red 81, Benzoic acid methylester, Carbendazim, Xylene, Ethylbenzene, Toluene, Naphthalene, 1,2,3-trichlorobenzene, Pentachlorophenol, Phenol, N,N-dimethylbenzamide, 3,5-dinitrobenzamide, N-methylbenzamide, Benzamide, phenanthrene, DDT, were chosen having Koc value range from 1.25 to 5.63. The Log Koc value was determined to be 1.835 ± 0.002at 25°C. Thus based on the result it is concluded that the test substance 3-methyl-1-phenylpyrazol-5-ylamine has a low sorption to soil and sediment and therefore has moderate migration potential to ground water.