4-aminomethyl-2-methoxyphenol hydrochloride

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Version / remarks:

July 17, 1992

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

4-aminomethyl-2-methoxyphenol hydrochloride

EC Number:

230-468-3

EC Name:

4-aminomethyl-2-methoxyphenol hydrochloride

Cas Number:

7149-10-2

Molecular formula:

C8H11NO2.ClH

IUPAC Name:

4-aminomethyl-2-methoxyphenol hydrochloride

Test material form:

other: solid

Details on test material:

- Physical state: white solid
- Analytical purity: 99.77 %(w/w)
- Lot/batch No.: 77

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, adapted

Remarks:

washed twice

Details on inoculum:

sewage supplied from plant in Groß-Zimmern, Germany; washed sludge suspension (4g/L) was aerated until use.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

4.12 mg/L

Based on:

ThOD

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium:
- Additional substrate:
- Solubilising agent (type and concentration if used):
- Test temperature:
- pH:
- pH adjusted: yes/no
- CEC (meq/100 g):
- Aeration of dilution water:
- Suspended solids concentration:
- Continuous darkness: yes/no
- Other:

TEST SYSTEM
- Culturing apparatus:
- Number of culture flasks/concentration:
- Method used to create aerobic conditions:
- Method used to create anaerobic conditions:
- Measuring equipment:
- Test performed in closed vessels due to significant volatility of test substance:
- Test performed in open system:
- Details of trap for CO2 and volatile organics if used:
- Other:

SAMPLING
- Sampling frequency:
- Sampling method:
- Sterility check if applicable:
- Sample storage before analysis:
- Other:

CONTROL AND BLANK SYSTEM
- Inoculum blank:
- Abiotic sterile control:
- Toxicity control:
- Other:

STATISTICAL METHODS:TEST CONDITIONS
- Composition of medium:
- Additional substrate:
- Solubilising agent (type and concentration if used):
- Test temperature: 22°C
- pH: 7.5
- pH adjusted: no
- CEC (meq/100 g):
- Aeration of dilution water: 8.8mg O2/L
- Suspended solids concentration:
- Continuous darkness: yes
- Other:

TEST SYSTEM
- Culturing apparatus: Karlsruher flasks 250mL
- Number of culture flasks/concentration:
- Method used to create aerobic conditions:
- Method used to create anaerobic conditions:
- Measuring equipment: WTW Oxi 340, Weilheim Germany
- Test performed in closed vessels due to significant volatility of test substance:
- Test performed in open system:
- Details of trap for CO2 and volatile organics if used:
- Other:

SAMPLING
- Sampling frequency:
- Sampling method:
- Sterility check if applicable:
- Sample storage before analysis:
- Other:

CONTROL AND BLANK SYSTEM
- Inoculum blank: 2.5mL of filtered inoculum added to 5L aqueous test medium
- Abiotic sterile control:
- Toxicity control: 20mg Vanillyamin-HCl and 10.2mg Aniline mixed into 5L of aqueous test medium
- Other:

STATISTICAL METHODS:

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

49

Sampling time:

28 d

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

Under the test coditions Vanillylamin-HCl was degraded by 49% baeed on ThOD (NH4) after 28 days of incubation. Since biodegradation did not exceed 60% by the end of the test, the substance cannot be considered readily biodegradable.

Executive summary:

Vanillylamin-HCl was investigated for its ready biodegradability in a Closed Bottle Test over 28 days. The biodegradation was followed by the uptake of oxygen of the micro organisms during exposure. As reference aniline was tested simultaneously under the same conditions as the test system.