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EC number: 206-376-4 | CAS number: 334-48-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 24 May - 27 May 1999
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- calculation of results based on nominal values despite >20% deviation from measured values
- GLP compliance:
- yes
- Remarks:
- according to the Japanese GLP Standard
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Vapour pressure: 1 mm at 125 °C
- Water solubility: < 1 mg/L
- log Pow: 4.09 - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0, 1.8, 3.2, 5.6, 10 and 18 mg/L (nominal)
- Sampling method: At study initiation: test solution for analytic was separately prepared, since the volume was > 5% of test water in vessels. After exposure: test solution was sampled directly from each test vessel.
- Sample storage conditions before analysis: Analysis was conducted immediately after sampling, no storage. - Vehicle:
- yes
- Details on test solutions:
- - Method: The test substance was dissolved in vehicle, and this stock solution was diluted in dilution water and stirred for 1 hour.
- Controls: dilution water with/without vehicle
- Chemical name of vehicle: dimethylformamide
- Concentration of vehicle in test medium: 0.1 mL/L
- Evidence of undissolved material: not observed - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: alga
- Strain: ATCC22662
- Source: American Type Culture Collection
- Age of inoculum: within 72 hours
- Method of cultivation: on rotary shaker (100 rpm)
ACCLIMATION
- Acclimation period: 3 days
- Culturing media and conditions: same as test - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 23.7 - 23.8 °C (23 ± 2 °C)
- pH:
- at the initiation: 7.6 - 7.8
at the end: 7.4 - 7.6 - Nominal and measured concentrations:
- nominal concentration: 1.8, 3.2, 5.6, 10, 18 mg/L
measured concentration: at test initiation: 1.8, 3.0, 5.9, 8.9, 16 mg/L
at test end (percent of nominal): 0.52 (29%), 1.2 (38%), 3.2 (57%), 6.0 (60%), 14 (78%) mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 500 mL Erlenmeyer flask with an air-permeable stopper
- Type: open
- Material, fill volume: glass, 100 mL
- Initial cells density: 10000 cells/mL
- Control end cells density: 2620000 ± 130000 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- No. of vessels per vehicle control (replicates): 3
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: not stated
- Intervals of water quality measurement: pH (0 and 72 hours), temperature, light intensity and number of revolutions (0, 24, 48, 72 hours) were determined.
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: not adjusted
- Photoperiod: continuously
- Light intensity and quality: 4300 - 5000 lux
EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations: electronic particle counter; 24, 48, 72 hours
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Range finding study: performed
- Test concentrations: 2.0 and 20 mg/L
- Results used to determine the conditions for the definitive study: 2.0 mg/L: growth inhibition was not observed, 20 mg/L: 93 % growth inhibition was observed - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate (reagent grade)
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 15 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 14 - 17 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 5.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 5.5 - 6.3 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EbC50: 0.44 mg/L - Reported statistics and error estimates:
- Barlett, Probit and Dunnett test
Reference
Table 1: Cell density of Selenastrum capricornutum after treatment with decanoic acid
Loading Rate (mg/L) |
Areal Mean Measured Conc. (mg/L) |
Cell Densities (cells/mL) |
||||
|
0 Hour |
24 Hour |
48 Hour |
72 Hour |
||
Control |
- |
Mean |
10000 |
65300 |
407000 |
2620000 |
SD |
0 |
5600 |
12000 |
130000 |
||
Solvent Control |
- |
Mean |
1000 |
66900 |
411000 |
2550000 |
SD |
0 |
3200 |
14000 |
190000 |
||
1.8 |
2.12 |
Mean |
1000 |
64000 |
392000 |
2630000 |
SD |
0 |
7200 |
8000 |
60000 |
||
3.2 |
|
Mean |
1000 |
54000 |
321000 |
2090000 |
|
SD |
0 |
3400 |
23000 |
50000 |
|
5.6 |
|
Mean |
1000 |
46800 |
239000 |
1260000 |
|
SD |
0 |
1700 |
10000 |
80000 |
|
10 |
|
Mean |
1000 |
33000 |
128000 |
465000 |
|
SD |
0 |
1900 |
8000 |
18000 |
|
18 |
|
Mean |
1000 |
23100 |
47000 |
99700 |
|
SD |
0 |
1200 |
3500 |
6800 |
Table 2: Growth inhibition of Selenastrum capricortum after treatment with decanoic acid
Nominal concentration (mg/L) |
Inhibition (%) Biomass |
Inhibition (%) Growth rate |
Control |
0 |
0 |
Solvent Control |
1.7 |
1.5 |
1.8 |
0.6 |
-0.7 |
3.2 |
20.4 |
0.9 |
5.6 |
49.3 |
10.9 |
10 |
79.0 |
28.3 |
18 |
94.6 |
60.4 |
Description of key information
ErC50 (72 h) = 15 mg/L (nominal, growth rate, OECD 201)
NOErC (72 h) = 3.2 mg/L (nominal, growth rate, OECD 201)
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 15 mg/L
- EC10 or NOEC for freshwater algae:
- 3.2 mg/L
Additional information
One study investigating the toxicity of decanoic acid (CAS 334-48-5) to freshwater algae is available.
The study (1999) was conducted according to OECD 201 and GLP. The test organism Pseudokirchneriella subcapitata was exposed to 5 nominal test item concentrations of 0 (control), 1.8, 3.2, 5.6, 10, 18 mg/L for 72 h. The substance was dissolved in dimethylformamide and monitored during the experiment by GC-MS analysis. While recoveries of the test item concentrations were above 80% of nominal at the start of the experiment, they decreased to 29 – 78% of the nominal values at the end of the experiment. The test showed growth rate inhibitions and resulted to an ErC50 (72 h) of 15 mg/L and an NOEC (72 h) of 3.2 mg/L (nominal).
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