azido(trioctyl)stannane

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2003

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

The test material is a mixture of Stannane, azidotrioctyl- and toluene (in 40- 60 % toluene solution).

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Daphnia magna

Details on test organisms:

Species: Daphnia magna (Straus), clone 5
Age at Test Start: 6-23.5 hours old
Sex: female
Origin: supplied 1997 by the Umweltbundesamt, Institut fur Wasser-,Boden- und Lufthygiene, Berlin, Germany
Breeding Conditions: The Daphnia were bred in the laboratories of IBACON under similar temperature and light conditions as in the test, and in reconstituted water of a similar quality regarding to pH, components of the main ions and total hardness as the test water used in the test (see below). The test organisms were not first brood progeny. The Daphnia of the stock culture were fed at least each working day with green algae (Desmodesmus subspicatus) freshly grown in the laboratories of IBACON.

Test type:

static

Water media type:

other: deionised water

Limit test:

yes

Total exposure duration:

48 h

Remarks on exposure duration:

The behaviour of the test item in test water was determined at the start of the test, after 24 and 48 hours test duration in all test concentrations.

Hardness:

CaCl2 x 2H2O 2.0 mmol/L (= 294.0 mg/L)
MgSO4 x 7H2O 0.5 mmol/L (= 123.0 mg/L)

Test temperature:

20°C

pH:

7.8- 8.0

Dissolved oxygen:

At the end of the test the dissolved oxygen concentration in the test media was >2 mg O2/L in the control and test vessels.

Salinity:

In deionised water (conductivity < 5 microS/cm) analytical grade salts were added to following nominal concentrations:
CaCl2 x 2H2O 2.0 mmol/L (= 294.0 mg/L)
MgSO4 x 7H2O 0.5 mmol/L (= 123.0 mg/L)
NaHCO3 0.75 mmol/L (= 65.0 mg/L)
KCl 0.075 mmol/L (= 5.8 mg/L)
Water Hardness 2.5 mmol/L (= 250.0 mg/L) as CaCO3
Alkalinity 0.8 mmol/L
Ratio of Ca : Mg = 4 : 1 (based on molarity)
Na : K = 10 : 1 (based on molarity)

Nominal and measured concentrations:

0.10, 0.21, 0.46, 1.0, 2.1, 4.6, 10, 21, 46 and 100 mg test item/L and a control.

Details on test conditions:

Light Regime: 16 h light; 8 h dark
Light Intensity: 450-490 lux

Reference substance (positive control):

not required

Remarks:

The experiment is valid because the mortality of Daphnia magna in the control was 0 % and no Daphnia were trapped at the water surface.

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.63 mg/L

Nominal / measured:

nominal

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

0.1 mg/L

Nominal / measured:

nominal

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

2.1 mg/L

Nominal / measured:

nominal

Details on results:

After 48 hours of exposure the toxicity of the test item to Daphnia magna had increased. In the control and in the test concentration of nominal 0.1 mg test item/L no immobility or mortality of the test animals or other signs of intoxication were determined during tbe test period of 48 hours. At the test concentration of 0.21 mg/L one Daphnia was immobile, at tbe test concentration of 0.46 mg/L 6 Daphnia were immobile and at the test concentration of 1.0 mg/L 15 Daphnia were immobile after 48 hours test duration. At the test concentration from 2.1 to 100 mg/L all Daphnia were immobile at the end of the test.
The 48-hour EC 50 was calculated to be 0.63 mg test item/L with 95 % confidence limits from 0.48 to 0.82 mg/L. The 48-hour ECO was 0.1 mg/L. The 48-hour EC 100 amounted to 2.1 mg test item/L.

Reported statistics and error estimates:

The 24-hour and 48-hour EC 50 and the 95 % confidence limits were calculated by the Moving average interpolation, using Critical Values Easy Essay Version 3.01. The EC0 and EC100 were determined directly from the raw data.

Validity criteria fulfilled:

yes

Conclusions:

The 48-hour EC 50 was calculated to be 0.63 mg test item/L with 95 % confidence limits from 0.48 to 0.82 mg/L. The 48-hour EC0 was 0.1 mg/L. The 48-hour EC 100 amounted to 2.1 mg test item/L.

Executive summary:

The influence of the test item TOTA (solution) on the mobility respectively survival of Daphnia magna was tested. Young Daphnia were exposed in a static test to the test item for 48 hours, added to water at a range of concentrations.

After 48 hours of exposure the toxicity of the test item to Daphnia magna had increased. In the control and in the test concentration of nominal 0.1 mg test item/L no immobility or mortality of the test animals or other signs of intoxication were determined during the test period of 48 hours. At the test concentration of 0.21 mg/L one Daphnia was immobile, at the test concentration of 0.46 mg/L 6 Daphnia were immobile and at the test concentration of 1.0 mg/L 15 Daphnia were immobile after 48 hours test duration. At the test concentration from 2.1 to 100 mg/L all Daphnia were immobile at the end of the test.

The 48-hour EC 50 was calculated to be 0.63 mg test item/L with 95 % confidence limits from 0.48 to 0.82 mg/L. The 48-hour EC0 was 0.1 mg/L. The 48-hour EC 100 amounted to 2.1 mg test item/L.

Description of key information

The influence of the test item TOTA (solution) on the mobility respectively survival of Daphnia magna was tested. Young Daphnia were exposed in a static test to the test item for 48 hours, added to water at a range of concentrations.

After 48 hours of exposure the toxicity of the test item to Daphnia magna had increased. In the control and in the test concentration of nominal 0.1 mg test item/L no immobility or mortality of the test animals or other signs of intoxication were determined during the test period of 48 hours. At the test concentration of 0.21 mg/L one Daphnia was immobile, at the test concentration of 0.46 mg/L 6 Daphnia were immobile and at the test concentration of 1.0 mg/L 15 Daphnia were immobile after 48 hours test duration. At the test concentration from 2.1 to 100 mg/L all Daphnia were immobile at the end of the test.

The 48-hour EC 50 was calculated to be 0.63 mg test item/L with 95 % confidence limits from 0.48 to 0.82 mg/L. The 48-hour EC0 was 0.1 mg/L. The 48-hour EC 100 amounted to 2.1 mg test item/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.63 mg/L