Benzenecarboximidamide, 4-amino-N-hydroxy-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guidline study under GLP-conditions, with minor or no deviations.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

4-amino-N-hydroxybenzene-1-carboximidamide

EC Number:

664-406-5

Cas Number:

277319-62-7

Molecular formula:

C7 H9 N3 O

IUPAC Name:

4-amino-N-hydroxybenzene-1-carboximidamide

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Samples was taken in triplicate from all test concentrations and the control at 0 h and 48 h. With every sampling 2 x 4 mL was taken and filled
into 4 mL glass vials. Samples were kept in the refrigerator at 5 +/-3°C until being sent to the analytical laboratory. Additional retain
samples (1 x 4 mL) were kept in the refrigerator until finalization of the study. Proper departure and arrival of the samples was documented.

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Common name: Daphnia magna Straus
Source: German Federal Environmental Agency, department V 3.2, Berlin
The current breeding has been held at Hydrotox GmbH since October 2012. Twice a week the animals are placed into fresh Elendt medium M4
(according to OECD 202). They are kept at approximately 10 animals / 200 mL. Daily, they are fed with a suspension of the algae Desmodesmus
subspicatus in order to keep the C-content in the incubation beakers at 0.1 mg C per Daphnia and day. The Ccontent of the algal suspension is
measured photometrical. The daphnids are held at 20 ± 2°C with a light : dark cycle of 16 : 8 h. According to OECD 202 daphnids aged less than 24
h should be tested. In the present test animals of an age of maximal 21.25 h were used.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC10

Effect conc.:

3.48 mg/L

Nominal / measured:

estimated

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

5.94 mg/L

Nominal / measured:

estimated

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

2.19 mg/L

Nominal / measured:

estimated

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95%-CL: 1.53 – 2.71 mg/L

Effect concentrations 4

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

3.96 mg/L

Nominal / measured:

estimated

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95%-CL:3.31 – 4.75 mg/L

Effect concentrations 5

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

<= 5.6 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 6

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

<= 3.1 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Reported statistics and error estimates:

The statistical evaluation was performed with the software ToxRatPro (Version 2.10.05, 02/2010, ToxRat solutions, Alsdorf, Germany). NOEC,
LOEC and EC50 were determined as far as mathematical possible.